Boissonneault G A, Harrison D E
Department of Clinical Sciences, University of Kentucky, Lexington 40536-0080.
J Nutr. 1994 Sep;124(9):1639-46. doi: 10.1093/jn/124.9.1639.
The objective of this study was to investigate food restriction-related changes in several indices of immune competence in young (11 wk old) and adult (33 wk old) female lean (+/?) and obese (ob/ob) C57BL/6J mice. Body weight accumulation, tail length accretion and organ weights were more severely curtailed by food restriction in obese mice than in lean mice. Tail collagen denaturation time increased with age, although the magnitude was greater in obese mice, and this change was minimized by food restriction. Splenocyte mitogen responses were generally not altered with age in lean or obese mice, whereas food restriction augmented these responses in lean mice while having no effect or reducing them in obese mice. The concanavalin A and phytohemagglutinin responses of splenocytes from young and adult obese mice were greater than those for lean mice, whereas the bacterial lipopolysaccharide response was elevated only in adult obese vs. lean mice. Flow cytometric analysis of splenocytes revealed an increase in Thy-1+ cells with food restriction vs. freely fed obese and lean mice, with a proportional decrease in Ig+ cells. Percentages of CD4+ and CD8+ cells increased with food restriction in both lean and obese mice. These results suggest that genetic obesity largely eliminates the immunopotentiating effects of food restriction, although the rate of "aging" may be reduced by food restriction.
本研究的目的是调查食物限制对年轻（11周龄）和成年（33周龄）雌性瘦型（+/？）和肥胖型（ob/ob）C57BL/6J小鼠免疫能力的几个指标的影响。与瘦型小鼠相比，肥胖小鼠的体重增加、尾巴长度增长和器官重量受食物限制的影响更为严重。尾巴胶原蛋白变性时间随年龄增加，尽管肥胖小鼠的变化幅度更大，而食物限制使这种变化最小化。在瘦型或肥胖型小鼠中，脾细胞丝裂原反应一般不会随年龄改变，而食物限制增强了瘦型小鼠的这些反应，对肥胖小鼠则无影响或使其降低。年轻和成年肥胖小鼠脾细胞对刀豆球蛋白A和植物血凝素的反应大于瘦型小鼠，而细菌脂多糖反应仅在成年肥胖小鼠与瘦型小鼠相比时升高。对脾细胞进行流式细胞术分析发现，与自由进食的肥胖和瘦型小鼠相比，食物限制使Thy-1+细胞增加，Ig+细胞成比例减少。在瘦型和肥胖型小鼠中，食物限制均使CD4+和CD8+细胞的百分比增加。这些结果表明，遗传性肥胖在很大程度上消除了食物限制的免疫增强作用，尽管食物限制可能会降低“衰老”速度。
