Kawanishi T, Kawanishi M, Ohata H, Toyoda K, Takahashi M, Momose K, Hayashi Y
Division of Pathology, National Institute of Health Sciences, Tokyo, Japan.
Jpn J Pharmacol. 1994 May;65(1):59-62. doi: 10.1254/jjp.65.59.
The relationship between spontaneous Ca2+ oscillations and cell proliferation was investigated in cultured longitudinal muscle cells from guinea pig ileum. BAPTA, caffeine, thapsigargine and La3+ suppressed the spontaneous Ca2+ oscillations and the DNA synthesis at 5.0 mM, 10.0 mM, 15.62 nM and 0.5 mM, respectively, whereas verapamil, nicardipine and ryanodine did not suppress either. However, BAPTA and La3+ did not inhibit the DNA synthesis at 1.25 mM and 0.13 mM, but did suppress the Ca2+ oscillations even at 1.0 mM and 0.1 mM, respectively. These results show that the spontaneous Ca2+ oscillations are not prerequisite for the cell proliferation.
