Verger J M, Grayon M, Gravouil F
Ann Rech Vet. 1975;6(2):187-205.
Two hundred and eight strains of Brucella isolated from cattle and 5 isolated from man in various areas of France, during a two-year period from 1972 to 1974, were examined by all methods recommended by the Subcommittee on Taxonomy of the genus Brucella and were found to have the same characteristics that identify and define the species Brucella abortus. Of the 213 strains, 105 (49,29 p. 100) were biotype I, 103 strains (48,36 p. 100) biotype 3, three strains (1,41 p. 100) biotype 4 and 2 strains (0,94 p. 100) biotype 2. The distribution of Brucella abortus biotypes isolated from cattle and man in France is reviewed and compared with the data from our sample. It corroborates the epidemiologic importance of Brucella abortus biotype 3, just after type 1, and the absence of biotypes 5 to 9(1) in France. Five strains of Brucella abortus biotype 3, originating from a well-defined geographical area, are ureaseless. This new biochemical character distinguishes these strains from the main group of biotype 3 and is discussed from an epidemiological and methodological point of view. Data on the oxydative rates obtained on 12 substrates by the 213 strains are presented in graphic form which reveals a metabolic profile for the species Brucella abortus. The interest of this graphic presentation is discussed and its use should be recommended.
1972年至1974年的两年间，从法国不同地区的牛身上分离出208株布鲁氏菌，从人身上分离出5株。采用布鲁氏菌属分类小组委员会推荐的所有方法对这些菌株进行检测，发现它们具有鉴定和定义流产布鲁氏菌物种的相同特征。在这213株菌株中，105株（49.29%）为生物型I，103株（48.36%）为生物型3，3株（1.41%）为生物型4，2株（0.94%）为生物型2。本文回顾了法国从牛和人身上分离出的流产布鲁氏菌生物型的分布情况，并与我们样本中的数据进行了比较。这证实了流产布鲁氏菌生物型3仅次于生物型1的流行病学重要性，以及法国不存在生物型5至9(1)的情况。来自一个明确地理区域的5株流产布鲁氏菌生物型3菌株无尿素酶活性。这一新的生化特征将这些菌株与生物型3的主要群体区分开来，并从流行病学和方法学角度进行了讨论。以图表形式呈现了213株菌株在12种底物上的氧化率数据，揭示了流产布鲁氏菌物种的代谢谱。讨论了这种图表呈现方式的意义，并建议予以使用。
