Gannon R L, Rea M A
Circadian Neurobiology Research Group, Armstrong Laboratory/CFTO, Brooks AFB, TX 78235.
Brain Res Mol Brain Res. 1994 Jun;23(4):338-44. doi: 10.1016/0169-328x(94)90244-5.
Eleven oligonucleotides directed against mRNA for AMPA, NMDA and metabotropic glutamate receptor subtypes were hybridized to rat coronal brain sections containing the suprachiasmatic nucleus (SCN). These oligonucleotides were hybridized to tissue samples collected at midday and midnight phases of the circadian cycle. Glutamate receptor mRNA for the AMPA subunits GluR1, GluR2 and GluR4, and the NMDA receptor subtype NMDAR1, were heavily expressed in the SCN and surrounding areas. The mRNA for the metabotropic glutamate subunit mGluR1 was only lightly expressed in the SCN. In contrast, mRNA for NMDAR2A, NMDAR2B, NMDAR2C and GluR3 was not detected in the SCN. The mRNA found to be expressed in the rat SCN was similar in samples collected at midday and midnight, suggesting no circadian variation in endogenous SCN glutamate receptors at these two times of the light-dark cycle.
针对AMPA、NMDA和代谢型谷氨酸受体亚型的mRNA的11种寡核苷酸与含有视交叉上核（SCN）的大鼠冠状脑切片进行杂交。这些寡核苷酸与在昼夜节律周期的中午和午夜阶段收集的组织样本进行杂交。AMPA亚基GluR1、GluR2和GluR4以及NMDA受体亚型NMDAR1的谷氨酸受体mRNA在SCN及其周围区域大量表达。代谢型谷氨酸亚基mGluR1的mRNA在SCN中仅轻度表达。相比之下，在SCN中未检测到NMDAR2A、NMDAR2B、NMDAR2C和GluR3的mRNA。在中午和午夜收集的样本中，发现大鼠SCN中表达的mRNA相似，这表明在明暗周期的这两个时间点，内源性SCN谷氨酸受体没有昼夜变化。
