用去污剂去除牛肝中磷脂酰胆碱交换蛋白的脂质。

Delipidation of the phosphatidylcholine exchange protein from beef liver by detergents.

Kamp H H, Wirtz K W, Van Deenen L L

Biochim Biophys Acta. 1975 Sep 19;398(3):401-14. doi: 10.1016/0005-2760(75)90191-5.

Abstract

A method is described to introduce [14C] phosphatidylcholine into the phosphatidylcholine exchange protein from beef liver. The effects of various detergents on this 14C-labelled phospholipid - protein complex are considered. 2. As shown by spectrophotometric and radioactivity analysis of polyacrylamide gels, sodium deoxycholate, Triton X-100, lysophosphatidylcholine and sodium dodecyl sulfate delipidate the exchange protein, while mixed phosphatidylcholine-detergents micelles are formed. 3. Protein delipidated by sodium deoxycholate, Triton X-100 and lysophosphatidylcholine retains its ability to catalyze the transfer of phosphatidylcholine between membranes. The immunological properties are similar to those of native protein as shown by double immunodiffusion in agar against an antiserum gamma-globulin fraction. 4. Sodium dodecyl sulfate and cetyltrimethylammonium bromide interact very strongly with the protein conferring their charge to the complex and destroying the antigenic determinants.

摘要

本文描述了一种将[14C]磷脂酰胆碱引入牛肝磷脂酰胆碱交换蛋白的方法。研究了各种去污剂对这种14C标记的磷脂 - 蛋白复合物的影响。2. 聚丙烯酰胺凝胶的分光光度法和放射性分析表明，脱氧胆酸钠、Triton X - 100、溶血磷脂酰胆碱和十二烷基硫酸钠可使交换蛋白脱脂，同时形成混合的磷脂酰胆碱 - 去污剂胶束。3. 经脱氧胆酸钠、Triton X - 100和溶血磷脂酰胆碱脱脂的蛋白保留了催化磷脂酰胆碱在膜之间转移的能力。如在琼脂中针对抗血清γ-球蛋白组分进行的双向免疫扩散所示，其免疫学性质与天然蛋白相似。4. 十二烷基硫酸钠和十六烷基三甲基溴化铵与蛋白强烈相互作用，赋予复合物电荷并破坏抗原决定簇。