Llanes C, Couturier M, Asfeld L, Grimont F, Michel-Briand Y
Laboratoire de Bactériologie, CHU/Hôpital Jean Minjoz, Besançon, France.
Res Microbiol. 1994 Jan;145(1):17-25. doi: 10.1016/0923-2508(94)90063-9.
Replicon typing is the identification of plasmids by hybridization with specific DNA probes which contain the genes involved in plasmid maintenance. This new method has been used to classify plasmids into replicon (rep) groups which can often be correlated with incompatibility (Inc) groups. We studied 71 multiresistant Serratia marcescens strains with 19 rep probes constructed from reference plasmid replicons belonging to known Inc groups. These probes are known to react with enteric bacterial plasmids. However, they did not represent the totality of the thirty known Inc groups. For 52% of the studied strains, plasmids were identified and classified into groups FIB, FIC, FIIA, HI2, L/M, N, B/O, P, W, Y and Com9. Most (79%) of the plasmid preparations hybridized with a single rep probe, and 21% hybridized with two different probes. Electrophoretic analysis of DNA suggested that double hybridization could result from the presence of either two different Inc plasmids in the same strain (e.g. S37) or one single plasmid with a multireplicon (e.g. S113).
复制子分型是通过与特定DNA探针杂交来鉴定质粒，这些探针包含参与质粒维持的基因。这种新方法已被用于将质粒分类为复制子（rep）组，这些组通常可与不相容性（Inc）组相关联。我们用从属于已知Inc组的参考质粒复制子构建的19种rep探针研究了71株多重耐药粘质沙雷氏菌菌株。已知这些探针可与肠道细菌质粒发生反应。然而，它们并不代表三十个已知Inc组的全部。对于52%的研究菌株，质粒被鉴定并分类为FIB、FIC、FIIA、HI2、L/M、N、B/O、P、W、Y和Com9组。大多数（79%）质粒制剂与单个rep探针杂交，21%与两种不同的探针杂交。DNA的电泳分析表明，双重杂交可能是由于同一菌株中存在两种不同的Inc质粒（如S37）或单个具有多复制子的质粒（如S113）所致。
