用于小牛血清中左旋咪唑酶法测定的单步提取程序的评估。

Evaluation of a single step extraction procedure for enzymatic assay of levamisole in calf serum.

作者信息

Ayyadevara S, Mercer J W, Snow L D

机构信息

Department of Chemistry, Louisiana Tech University, Ruston 71272.

出版信息

Res Commun Chem Pathol Pharmacol. 1994 May;84(2):245-52.

PMID:8091009

Abstract

A single step extraction procedure for recovery of the anthelmintic drug levamisole (LEV) from aqueous solutions and calf serum was evaluated. Levamisole was extracted from aqueous solutions and calf serum with 1.5 ml of ethyl acetate. After evaporation to dryness, the LEV content of extracts was estimated by measuring LEV inhibition of bovine milk fat globule membrane alkaline phosphatase. Lipid interference with absorbance readings was eliminated by the addition of 1.0 ml of chloroform to the assay mixtures. The recovery of LEV from both aqueous samples and serum samples by this single step extraction procedure coupled with enzymatic assay was 90%. The effective range for serum LEV determination was 0.3 to 20 micrograms/ml.

摘要

评估了一种从水溶液和小牛血清中回收驱虫药左旋咪唑（LEV）的单步提取程序。用1.5毫升乙酸乙酯从水溶液和小牛血清中提取左旋咪唑。蒸发至干后，通过测量左旋咪唑对牛乳脂肪球膜碱性磷酸酶的抑制作用来估计提取物中的左旋咪唑含量。通过向测定混合物中加入1.0毫升氯仿消除脂质对吸光度读数的干扰。通过这种单步提取程序结合酶促测定法从水性样品和血清样品中回收左旋咪唑的回收率为90%。血清左旋咪唑测定的有效范围为0.3至20微克/毫升。