Sasaki H, Yamaguchi T, Kumada K, Higashiyama H, Terasaki M, Mashima S, Mitsuyoshi A, Nakagami M, Morimoto T, Shimahara Y
Second Department of Surgery, Faculty of Medicine, Kyoto University, Japan.
Res Exp Med (Berl). 1994;194(3):139-45. doi: 10.1007/BF02576374.
The effect of low-molecular-weight heparin (LMWH) on hepatic mithocondrial metabolism was compared with that of unfractionated heparin (UH) after the intravenous administration of these two kinds of heparin to normal rabbits. The magnitude of decrease in blood triglyceride levels 5 min after administration of UH (200 U/kg) was significantly greater than after LMWH (200 U/kg). Free fatty acid levels in the blood were significantly higher after this dose of UH than after LMWH. Blood total ketone body levels (acetoacetate + 3-hydroxybutyrate) 15 min after injection of 50 U/kg of UH were significantly higher than those after a dose of 50 U/kg of LMWH, and levels after 200 U/kg of UH were significantly higher than those after 200 U/kg of LMWH at 15, 30, 45 and 60 min. Enhanced ketogenesis was not noted after LMWH at any of the doses, or after UH at 3 U/kg. Arterial ketone body ratio (AKBR; acetoacetate/3-hydroxybutyrate), which reflects the hepatic mitochondrial oxidation-reduction state (NAD+/NADH), was maintained above 1.0 in all groups except in the U-200 group, while AKBR in that group was significantly decreased to 0.99 +/- 0.14 at 30 min, and further decreased to 0.80 +/- 0.08 at 60 min. These results indicate that LMWH has less effect on lipolysis than UH and does not enhance ketogenesis, resulting in less deterioration of mitochondrial redox state.
将低分子量肝素（LMWH）和普通肝素（UH）静脉注射给正常家兔后，比较了它们对肝脏线粒体代谢的影响。注射UH（200 U/kg）后5分钟，血液甘油三酯水平的下降幅度显著大于注射LMWH（200 U/kg）后。该剂量的UH注射后血液中的游离脂肪酸水平显著高于LMWH注射后。注射50 U/kg UH后15分钟，血液总酮体水平（乙酰乙酸+3-羟基丁酸）显著高于注射50 U/kg LMWH后，而注射200 U/kg UH后15、30、45和60分钟时的水平显著高于注射200 U/kg LMWH后。在任何剂量的LMWH注射后以及3 U/kg UH注射后均未观察到生酮作用增强。反映肝脏线粒体氧化还原状态（NAD+/NADH）的动脉酮体比率（AKBR；乙酰乙酸/3-羟基丁酸）在除U-200组外的所有组中均维持在1.0以上，而该组的AKBR在30分钟时显著降至0.99±0.14，在60分钟时进一步降至0.80±0.08。这些结果表明，LMWH对脂肪分解的影响小于UH，且不会增强生酮作用，导致线粒体氧化还原状态的恶化程度较低。
