酵母Rad1-Rad10 DNA核酸内切酶对模型重组和修复中间体的特异性切割。

Specific cleavage of model recombination and repair intermediates by the yeast Rad1-Rad10 DNA endonuclease.

作者信息

Bardwell A J, Bardwell L, Tomkinson A E, Friedberg E C

机构信息

Laboratory of Molecular Pathology, University of Texas Southwestern Medical Center at Dallas 75235.

出版信息

Science. 1994 Sep 30;265(5181):2082-5. doi: 10.1126/science.8091230.

DOI:10.1126/science.8091230

PMID:8091230

Abstract

The RAD1 and RAD10 genes of Saccharomyces cerevisiae are required for both nucleotide excision repair and certain mitotic recombination events. Here, model recombination and repair intermediates were used to show that Rad1-Rad10-mediated cleavage occurs at duplex-single-strand junctions. Moreover, cleavage occurs only on the strand containing the 3' single-stranded tail. Thus, both biochemical and genetic evidence indicate a role for the Rad1-Rad10 complex in the cleavage of specific recombination intermediates. Furthermore, these data suggest that Rad1-Rad10 endonuclease incises DNA 5' to damaged bases during nucleotide excision repair.

摘要

酿酒酵母的RAD1和RAD10基因对于核苷酸切除修复和某些有丝分裂重组事件都是必需的。在此，利用模型重组和修复中间体表明，Rad1-Rad10介导的切割发生在双链-单链交界处。此外，切割仅发生在含有3'单链尾巴的链上。因此，生化和遗传证据均表明Rad1-Rad10复合物在特定重组中间体的切割中起作用。此外，这些数据表明，在核苷酸切除修复过程中，Rad1-Rad10内切酶在受损碱基的5'端切割DNA。