Cantoni O, Guidarelli A, Sestili P, Mannello F, Gazzanelli G, Cattabeni F
Istituto di Farmacologia e Farmacognosia, Università degli Studi di Urbino, Italy.
Toxicol Lett. 1994 Sep;73(3):193-9. doi: 10.1016/0378-4274(94)90058-2.
H2O2-sensitive and -resistant sublines of Chinese Hamster Ovary (CHO) cells were tested for their sensitivity to the growth inhibitory effect elicited by increasing concentrations of the oxidant under conditions of normal or reduced catalase activity. Experimental results have demonstrated that, under conditions of reduced catalase activity, the cytotoxic action of H2O2 was differentially regulated in resistant and sensitive cells. Indeed, the parental cell line and cells resistant to low concentrations of H2O2 (V 250 cells) depended on catalase to a lower extent than did highly resistant cells (V 850 cells). It is interesting to note that V 250 cells had more catalase, on a per million cell basis, than V 850 cells. We conclude that acquired resistance to oxidative stress is not entirely dependent on catalase and that the contribution of catalase depends on the degree of resistance to the oxidant.
对中国仓鼠卵巢（CHO）细胞的过氧化氢敏感和抗性亚系，在正常或过氧化氢酶活性降低的条件下，测试了它们对因氧化剂浓度增加所引发的生长抑制作用的敏感性。实验结果表明，在过氧化氢酶活性降低的条件下，过氧化氢对细胞的毒性作用在抗性细胞和敏感细胞中受到不同的调节。实际上，亲代细胞系和对低浓度过氧化氢有抗性的细胞（V 250细胞）对过氧化氢酶的依赖程度低于高度抗性细胞（V 850细胞）。有趣的是，以每百万细胞计，V 250细胞比V 850细胞具有更多的过氧化氢酶。我们得出结论，对氧化应激的获得性抗性并不完全依赖于过氧化氢酶，并且过氧化氢酶的作用取决于对氧化剂的抗性程度。
