Franco R S, Lee K N, Barker-Gear R, Gates R, Menitove J E
Hematology/Oncology Division, University of Cincinnati College of Medicine, Ohio.
Transfusion. 1994 Sep;34(9):784-9. doi: 10.1046/j.1537-2995.1994.34994378280.x.
The objectives of this research were 1) to determine whether two populations of platelets may be labeled with different levels of biotin and followed concurrently in vivo by flow cytometry and 2) to determine whether the level of biotinylation affects the in vivo platelet recovery and survival.
Two platelet aliquots were biotinylated under conditions that resulted in either a lower or a higher number of biotin molecules per platelet. After transfusion, the two populations were distinguished and quantitated by flow cytometry.
In five animals, recoveries were 69.8 +/- 27.0 percent for low-biotin platelets and 72.6 +/- 26.7 percent for high-biotin platelets. For each animal, the recoveries agreed closely. Life span, determined by the multiple-hit method, was 2.68 +/- 0.63 days for low-biotin platelets and 2.58 +/- 0.69 days for high-biotin platelets. These values for recovery and life span are consistent with those measured in rabbits by using radioisotope labels.
Platelet biotinylation offers a nonisotopic method for direct comparison of alternative harvest and storage conditions. It also offers the potential for simultaneous evaluation of the in vivo characteristics of platelets from at least two donors.
本研究的目的是：1）确定是否可以用不同水平的生物素标记两个血小板群体，并通过流式细胞术在体内同时追踪它们；2）确定生物素化水平是否会影响体内血小板的恢复和存活。
在导致每个血小板生物素分子数量较低或较高的条件下，对两份血小板样本进行生物素化。输血后，通过流式细胞术区分并定量这两个群体。
在五只动物中，低生物素血小板的回收率为69.8±27.0%，高生物素血小板的回收率为72.6±26.7%。对于每只动物，回收率非常接近。通过多次打击法确定的低生物素血小板寿命为2.68±0.63天，高生物素血小板寿命为2.58±0.69天。这些回收率和寿命值与使用放射性同位素标记在兔子身上测得的值一致。
血小板生物素化提供了一种非同位素方法，可直接比较不同的采集和储存条件。它还具有同时评估至少两个供体血小板体内特性的潜力。
