Murphy J E, Goff S P
Department of Biochemistry and Molecular Biophysics, Columbia University College of Physicians and Surgeons, New York, New York 10032.
Virology. 1994 Oct;204(1):458-61. doi: 10.1006/viro.1994.1554.
The integration of retroviral DNA to form the provirus requires the presence of short inverted repeat sequences at the termini of the linear viral DNA. We have previously described the construction of a mutant of Moloney murine leukemia virus, carrying an 8-bp deletion in the U5 terminus, that is defective in establishing the integrated provirus. We have now used a selectable marker to allow recovery of rare proviruses formed after transduction from NIH/3T3 cells by retroviral vectors carrying this mutation. Analysis of two such proviruses showed that both were recombinants between the vector and VL30 DNA and arose such that the VL30 sequences could provide an intact terminal sequence for integration.
逆转录病毒DNA整合形成前病毒需要线性病毒DNA末端存在短的反向重复序列。我们之前描述了莫洛尼鼠白血病病毒突变体的构建，该突变体在U5末端有一个8碱基对的缺失，在建立整合前病毒方面存在缺陷。我们现在使用一个选择标记来回收携带这种突变的逆转录病毒载体从NIH/3T3细胞转导后形成的罕见前病毒。对两个这样的前病毒的分析表明，它们都是载体和VL30 DNA之间的重组体，并且产生的方式使得VL30序列可以为整合提供完整的末端序列。
