Roberts P L, Walker C P, Feldman P A
Bio Products Laboratory, Elstree, UK.
Vox Sang. 1994;67 Suppl 1:69-71.
Virus reduction during the copper chelate affinity chromatography stage used during the purification of a new high-purity factor IX (BPLs 9MC) has been investigated. Virus reduction for the enveloped virus Sindbis was 6.5 log, a value which included approximately 2 log of inactivation due to the use of an acidic wash buffer (pH 4.4) during chromatography. In the case of the non-enveloped hepatitis-A-like poliovirus, which is acid-resistant, the virus reduction value was 4.0 log and was exclusively due to physical virus removal during the chromatographic process.
对一种新型高纯度凝血因子IX（BPLs 9MC）纯化过程中使用的铜螯合亲和色谱阶段的病毒去除情况进行了研究。包膜病毒辛德毕斯病毒的病毒去除率为6.5对数，该数值包括在色谱过程中使用酸性洗涤缓冲液（pH 4.4）导致的约2对数的失活。对于耐酸的非包膜甲型肝炎样脊髓灰质炎病毒，病毒去除率为4.0对数，这完全是由于色谱过程中病毒的物理去除。
