血清素诱导的猪空肠短路电流

Serotonin-induced short-circuit current in pig jejunum.

作者信息

Hansen M B, Thorbøll J E, Christensen P, Bindslev N, Skadhauge E

机构信息

Department of Anatomy and Physiology, Royal Veterinary and Agricultural University, Frederiksberg C, Denmark.

出版信息

Zentralbl Veterinarmed A. 1994 Mar;41(2):110-20. doi: 10.1111/j.1439-0442.1994.tb00073.x.

DOI:10.1111/j.1439-0442.1994.tb00073.x

PMID:8091886

Abstract

This paper presents the effects of serotonin (5-HT) on short-circuit current (SCC), sodium and chloride fluxes, and prostaglandin E2 release in pig jejunum, using the Ussing-chamber technique. 5-HT elicited a dose-dependent increase in SCC, yielding an EC50 of 6 +/- 4 microM and EMAX of 77 +/- 8 microA.cm-2 using about 100 microM. Inhibited sodium absorption and stimulated chloride secretion carried part of the 5-HT-induced SCC. 5-HT caused a dose-independent PGE2 release, and indomethacin reduced the SCC-inducing effect of 5-HT by 40%. Octreotide, a long-lasting somatostatin analogue, also reduced 5-HT-induced SCC by about 40%, while tetrodotoxin (TTX) did not alter the effect of 5-HT. In conclusion, 5-HT causes a dose-dependent indomethacin and octreotide-sensitive, and TTX-insensitive increase in SCC, and a chloride secretion and inhibited sodium absorption and an increased release of PGE2 in pig jejunum in vitro.

摘要

本文采用尤斯灌流小室技术，研究了血清素（5-羟色胺，5-HT）对猪空肠短路电流（SCC）、钠和氯通量以及前列腺素E2释放的影响。5-HT引起SCC呈剂量依赖性增加，使用约100微摩尔时，其半数有效浓度（EC50）为6±4微摩尔，最大效应（EMAX）为77±8微安/平方厘米。5-HT诱导的SCC部分是由抑制钠吸收和刺激氯分泌所致。5-HT引起前列腺素E2释放不依赖剂量，吲哚美辛可使5-HT诱导的SCC效应降低40%。长效生长抑素类似物奥曲肽也可使5-HT诱导的SCC降低约40%，而河豚毒素（TTX）不改变5-HT的效应。总之，5-HT可引起猪空肠SCC呈剂量依赖性增加，且对吲哚美辛和奥曲肽敏感、对TTX不敏感，还可导致氯分泌、抑制钠吸收以及增加前列腺素E2释放。