从牛气管软骨分离出的与纤调蛋白相连的硫酸角质素链的结构。角质酶消化产生的寡糖。
Structure of the keratan sulphate chains attached to fibromodulin isolated from bovine tracheal cartilage. Oligosaccharides generated by keratanase digestion.
作者信息
Lauder R M, Huckerby T N, Nieduszynski I A
机构信息
Division of Biological Sciences, University of Lancaster, Bailrigg, U.K.
出版信息
Biochem J. 1994 Sep 1;302 ( Pt 2)(Pt 2):417-23. doi: 10.1042/bj3020417.
Abstract
The structure of the repeat region and chain caps of the N-linked keratan sulphate chains attached to bovine tracheal cartilage fibromodulin has been examined. The chains were fragmented by keratanase digestion, the resultant oligosaccharides isolated by strong anion-exchange chromatography, and their structures determined using high-field 1H-n.m.r. spectroscopy. The chains were found to possess the following general structure: [formula: see text] All of the capping oligosaccharides isolated terminate with alpha(2-3)-linked N-acetylneuraminic acid. No alpha(2-6)-linked N-acetylneuraminic acid chain terminators, nor any fucose, alpha (1-3)-linked to N-acetylglucosamine along the repeat region, were detected. This work demonstrates that the structure of the repeat region and chain caps of N-linked keratan sulphate attached to fibromodulin isolated from bovine tracheal cartilage is identical with that of O-linked keratan sulphate chains attached to aggrecan derived from non-articular cartilage.
摘要
已对连接至牛气管软骨纤调蛋白的 N - 连接硫酸角质素链的重复区域结构和链端帽进行了研究。通过角质酶消化使这些链断裂，通过强阴离子交换色谱法分离得到的寡糖，并使用高场 1H - 核磁共振光谱法确定其结构。发现这些链具有以下一般结构：[公式：见正文] 所有分离得到的封端寡糖均以α(2 - 3)连接的 N - 乙酰神经氨酸结尾。未检测到α(2 - 6)连接的 N - 乙酰神经氨酸链终止剂，也未检测到沿重复区域与 N - 乙酰葡糖胺α(1 - 3)连接的任何岩藻糖。这项工作表明，从牛气管软骨分离的纤调蛋白上连接的 N - 连接硫酸角质素的重复区域结构和链端帽与从非关节软骨衍生的聚集蛋白聚糖上连接的 O - 连接硫酸角质素链的结构相同。
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