大鼠睾丸特异性钙调神经磷酸酶B亚基同工型β1在发育中大鼠睾丸的原位定位

In situ localization of rat testis-specific calcineurin B subunit isoform beta 1 in the developing rat testis.

作者信息

Miyamoto K, Matsui H, Tomizawa K, Kuwata Y, Itano T, Tokuda M, Hatase O

机构信息

Department of Physiology, Kagawa Medical School, Japan.

出版信息

Biochem Biophys Res Commun. 1994 Sep 15;203(2):1275-83. doi: 10.1006/bbrc.1994.2320.

DOI:10.1006/bbrc.1994.2320

PMID:8093044

Abstract

In situ localization and developmental changes in expression of testis-specific calcineurin B subunit isoform beta 1 was examined in rat testis. Two different sizes of mRNA signal, 4.0 kb and 0.9 kb, were detected by Northern blot hybridization. Both signals were expressed synchronously with the start of meiosis at 3 weeks after birth, and increased depending on the maturation of spermatogenesis. In situ hybridization using non-radioactive riboprobes showed that the beta 1 mRNA was specifically localized to spermatocytes where meiosis occurs but none or very little was observed in spermatogonia, spermatids, Sertoli or Leydig cells.

摘要

在大鼠睾丸中检测了睾丸特异性钙调神经磷酸酶B亚基同工型β1的原位定位及表达的发育变化。通过Northern印迹杂交检测到两种不同大小的mRNA信号，分别为4.0 kb和0.9 kb。两种信号均在出生后3周随着减数分裂的开始同步表达，并随着精子发生的成熟而增加。使用非放射性核糖探针的原位杂交显示，β1 mRNA特异性定位于发生减数分裂的精母细胞，而在精原细胞、精子细胞、支持细胞或间质细胞中未观察到或观察到极少。