Sagami H, Kikuchi A, Ogura K, Fushihara K, Nishino T
Institute for Chemical Reaction Science, Tohoku University, Sendai, Japan.
Biochem Biophys Res Commun. 1994 Sep 15;203(2):972-8. doi: 10.1006/bbrc.1994.2277.
Incorporation of [3H]mevalonic acid-derived materials into proteins was studied with extremely halophilic archaebacteria, Halobacterium halobium and Halobacterium cutirubrum. Several labeled proteins were detected on SDS-polyacrylamide gel electrophoresis followed by fluorography. The majority of the radioactive materials released from the labeled proteins by sulfonium salt cleavage moved with a mobility similar to that of a C85 polyprenol on reverse-phase thin-layer chromatography, and no radioactive farnesol was found on the chromatography. However, a weak but significant protein farnesyltransferase activity was detected in in vitro experiments with a combination of [3H]farnesyl diphosphate and Ras precursor protein.
用嗜盐古细菌盐生盐杆菌和红皮盐杆菌研究了[3H]甲羟戊酸衍生物质掺入蛋白质的情况。在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳后进行荧光自显影，检测到几种标记蛋白。通过锍盐裂解从标记蛋白中释放出的大多数放射性物质在反相薄层色谱上的迁移率与C85聚戊烯醇相似，色谱上未发现放射性法呢醇。然而，在体外实验中，用[3H]法呢基二磷酸和Ras前体蛋白组合检测到了微弱但显著的蛋白质法呢基转移酶活性。
