Heiling V J, Campbell P J, Gottesman I S, Tsalikian E, Beaufrere B, Gerich J E, Haymond M W
Department of Pediatrics, Mayo Clinic and Foundation, Rochester, MN 55905.
J Clin Endocrinol Metab. 1993 Jan;76(1):203-6. doi: 10.1210/jcem.76.1.8093620.
To determine the independent effects of insulin and glucose on the rate of appearance of leucine in humans, five normal volunteers were studied using a pancreatic clamp with sequential insulin infusions of 1.8, 3.6 and 7.2 pmol kg/min-1. On each of three separate occasions, the plasma glucose level was maintained at 5.0, 9.4 or 14 mM. The rate of appearance of leucine was similar at each insulin infusion rate regardless of the ambient glucose concentration or the glucose infusion rate. These studies demonstrate that during insulin and glucose infusion, insulin, but neither the plasma glucose concentration nor the rate of glucose utilization, primarily regulates leucine flux (a reflection of endogenous whole body proteolysis) in normal humans.
为了确定胰岛素和葡萄糖对人体亮氨酸出现率的独立影响，使用胰腺钳夹技术，对五名正常志愿者进行了研究，依次输注1.8、3.6和7.2 pmol kg/min-1的胰岛素。在三个不同的时间段里，每次都将血浆葡萄糖水平维持在5.0、9.4或14 mM。无论周围葡萄糖浓度或葡萄糖输注速率如何，在每个胰岛素输注速率下，亮氨酸的出现率都是相似的。这些研究表明，在输注胰岛素和葡萄糖期间，胰岛素而非血浆葡萄糖浓度或葡萄糖利用率，主要调节正常人的亮氨酸通量（反映内源性全身蛋白水解）。
