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在产生耐甲硫氨酸亚砜亚胺谷氨酰胺合成酶的枯草芽孢杆菌突变体中，谷氨酰胺合成酶调节基因（glnRA）操纵子的调控发生改变。

Altered regulation of the glnRA operon in a Bacillus subtilis mutant that produces methionine sulfoximine-tolerant glutamine synthetase.

作者信息

Schreier H J, Rostkowski C A, Kellner E M

机构信息

Center of Marine Biotechnology, University of Maryland, Baltimore 21202.

出版信息

J Bacteriol. 1993 Feb;175(3):892-7. doi: 10.1128/jb.175.3.892-897.1993.

DOI:10.1128/jb.175.3.892-897.1993
PMID:8093698
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC196239/
Abstract

A Bacillus subtilis mutant that produced glutamine synthetase (GS) with altered sensitivity to DL-methionine sulfoximine was isolated. The mutation, designated glnA33, was due to a T.A-to-C.G transition, changing valine to alanine at codon 190 within the active-site C domain. Altered regulation was observed for GS activity and antigen and mRNA levels in a B. subtilis glnA33 strain. The mutant enzyme was 28-fold less sensitive to DL-methionine sulfoximine and had a 13.0-fold-higher Km for hydroxylamine and a 4.8-fold-higher Km for glutamate than wild-type GS did.

摘要

分离出了一株枯草芽孢杆菌突变体，该突变体产生的谷氨酰胺合成酶（GS）对DL-甲硫氨酸亚砜亚胺的敏感性发生了改变。该突变被命名为glnA33，是由T.A到C.G的转换引起的，导致活性位点C结构域内第190位密码子处的缬氨酸变为丙氨酸。在枯草芽孢杆菌glnA33菌株中观察到GS活性、抗原和mRNA水平的调节发生了改变。与野生型GS相比，突变酶对DL-甲硫氨酸亚砜亚胺的敏感性降低了28倍，对羟胺的Km值高13.0倍，对谷氨酸的Km值高4.8倍。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a885/196239/e55a345c3e74/jbacter00045-0327-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a885/196239/e55a345c3e74/jbacter00045-0327-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a885/196239/e55a345c3e74/jbacter00045-0327-a.jpg

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本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Glutamine synthetase gene of Bacillus subtilis.枯草芽孢杆菌的谷氨酰胺合成酶基因
Gene. 1984 Dec;32(3):427-38. doi: 10.1016/0378-1119(84)90018-0.
3
Bacillus subtilis glutamine synthetase mutants pleiotropically altered in glucose catabolite repression.枯草芽孢杆菌谷氨酰胺合成酶突变体在葡萄糖分解代谢阻遏中发生多效性改变。
莱茵衣藻利用L-蛋氨酸-S-亚砜亚胺和草丁膦作为氮源生长的突变体的特性分析。
Plant Physiol. 1996 Apr;110(4):1215-1222. doi: 10.1104/pp.110.4.1215.
4
beta-Glutamate as a substrate for glutamine synthetase.β-谷氨酸作为谷氨酰胺合成酶的底物。
Appl Environ Microbiol. 2001 Oct;67(10):4458-63. doi: 10.1128/AEM.67.10.4458-4463.2001.
5
Functional analysis of 14 genes that constitute the purine catabolic pathway in Bacillus subtilis and evidence for a novel regulon controlled by the PucR transcription activator.枯草芽孢杆菌中构成嘌呤分解代谢途径的14个基因的功能分析以及由PucR转录激活因子控制的新型调控子的证据。
J Bacteriol. 2001 Jun;183(11):3293-302. doi: 10.1128/JB.183.11.3293-3302.2001.
6
Nitrogen regulation of nasA and the nasB operon, which encode genes required for nitrate assimilation in Bacillus subtilis.枯草芽孢杆菌中编码硝酸盐同化所需基因的nasA和nasB操纵子的氮调控。
J Bacteriol. 1995 Feb;177(3):573-9. doi: 10.1128/jb.177.3.573-579.1995.
J Bacteriol. 1984 Feb;157(2):612-21. doi: 10.1128/jb.157.2.612-621.1984.
4
New class of Bacillus subtilis glutamine-requiring mutants.新型枯草芽孢杆菌谷氨酰胺需求突变体
J Bacteriol. 1981 Nov;148(2):653-8. doi: 10.1128/jb.148.2.653-658.1981.
5
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6
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Proc Natl Acad Sci U S A. 1981 Jun;78(6):3743-7. doi: 10.1073/pnas.78.6.3743.
7
Selection of Bacillus subtilis mutants impaired in ammonia assimilation.氨同化受损的枯草芽孢杆菌突变体的筛选。
J Bacteriol. 1980 Feb;141(2):985-8. doi: 10.1128/jb.141.2.985-988.1980.
8
Identification of L-methionine S-sulfoximine as the diastereoisomer of L-methionine SR-sulfoximine that inhibits glutamine synthetase.鉴定L-蛋氨酸S-亚砜亚胺为抑制谷氨酰胺合成酶的L-蛋氨酸SR-亚砜亚胺的非对映异构体。
Biochemistry. 1969 Jun;8(6):2681-5. doi: 10.1021/bi00834a066.
9
Some kinetic properties of Bacillus subtilis glutamine synthetase.枯草芽孢杆菌谷氨酰胺合成酶的一些动力学特性。
J Biol Chem. 1970 Oct 25;245(20):5206-13.
10
Bacillus subtilis glutamine synthetase. Purification and physical characterization.枯草芽孢杆菌谷氨酰胺合成酶。纯化及物理特性分析。
J Biol Chem. 1970 Oct 25;245(20):5195-205.