Huppi K, Siwarski D, Shaughnessy J D, Mushinski J F
Molecular Genetics Section, NCI/NIH, Bethesda, MD 20892.
Int J Cancer. 1993 Feb 1;53(3):493-8. doi: 10.1002/ijc.2910530323.
In a series of mouse pvt-1 cDNA clones prepared from an immortalized B-cell line that contains an amplified c-myc/pvt-1 region, we have identified a unique cDNA, AJ-I, which contains 57 bp of pvt-1 sequence (pvt-1a) spliced to a novel sequence of 2.1 kb. We report here that this 3' segment (termed AJ-IX) maps more than 60 kb telomeric to pvt-1a and is encoded by a novel locus (AJ-I) on mouse chromosome 15. The AJ-IX probe detects a transcript that is expressed only in normal mouse brain and testis. Several mast-cell tumors, Ly-I+ B-lymphocytic cell lines and a neuroblastoma also display abundant levels of AJ-IX-specific mRNA. However, splicing of pvt-1a to AJ-IX is found exclusively in Ly-I+ B-cell lines that contain amplified c-myc/pvt-1. We conclude that some features in the generation of this amplicon facilitates the synthesis of a pvt-1/AJ-IX chimeric mRNA that may play a role in immortalization of these Ly-I+ B-cell lines.
在一系列从小鼠永生B细胞系制备的pvt - 1 cDNA克隆中，该细胞系含有扩增的c - myc/pvt - 1区域，我们鉴定出一个独特的cDNA，AJ - I，它包含57 bp的pvt - 1序列（pvt - 1a）与一个2.1 kb的新序列拼接。我们在此报告，这个3' 片段（称为AJ - IX）定位于pvt - 1a端粒方向超过60 kb处，并且由小鼠15号染色体上的一个新基因座（AJ - I）编码。AJ - IX探针检测到一种仅在正常小鼠脑和睾丸中表达的转录本。一些肥大细胞瘤、Ly - I + B淋巴细胞系和一个神经母细胞瘤也显示出丰富水平的AJ - IX特异性mRNA。然而，pvt - 1a与AJ - IX的拼接仅在含有扩增的c - myc/pvt - 1的Ly - I + B细胞系中发现。我们得出结论，这个扩增子产生过程中的一些特征促进了pvt - 1/AJ - IX嵌合mRNA的合成，这可能在这些Ly - I + B细胞系的永生化中起作用。
