使用对K88菌毛a决定簇具有特异性的单克隆抗体检测猪的产肠毒素大肠杆菌。
Use of monoclonal antibodies specific for the a determinant of K88 pili for detection of enterotoxigenic Escherichia coli in pigs.
作者信息
Westerman R B, Fortner G W, Mills K W, Phillips R M, Greenwood J M
机构信息
Department of Veterinary Diagnostic Investigation, College of Veterinary Medicine, Kansas State University, Manhattan 66506.
出版信息
J Clin Microbiol. 1993 Feb;31(2):311-4. doi: 10.1128/jcm.31.2.311-314.1993.
Abstract
Monoclonal antibodies directed against the a determinant of K88 pili from porcine enterotoxigenic Escherichia coli which react with all three K88 variants have been produced. These antibodies have been used for diagnosis of porcine enterotoxigenic E. coli in a direct enzyme-linked immunosorbent assay with sensitivity to 50 ng of pilus protein per ml.
摘要
已制备出针对猪产肠毒素大肠杆菌K88菌毛a决定簇的单克隆抗体，该抗体可与所有三种K88变体发生反应。这些抗体已用于直接酶联免疫吸附试验诊断猪产肠毒素大肠杆菌，其灵敏度为每毫升50纳克菌毛蛋白。
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