Hsia S, Tong J Y, Parris G L, Nghiem D D, Cottington E M, Rudert W A, Trucco M
Department of Laboratory Medicine, Allegheny General Hospital, Pittsburgh, Pennsylvania.
Transplantation. 1993 Feb;55(2):395-9. doi: 10.1097/00007890-199302000-00030.
HLA DRB1 allelic types were determined by using sequence-specific oligonucleotides for the analysis of 91 renal donor-recipient pairs that were followed for 30 months. The Kaplan-Meier method was used to evaluate graft survivals (GS) of the matched and mismatched groups. The degree of compatibility was measured by allelic type matches and mismatches of the pairs. Furthermore, HLA DR genotypes were categorized into five groups, namely DR1, DR2, DR4, the group that also has the DRw52 allele, and the group of DR7, DR9, and DR10. All types within a group were considered a match. Serotypes of the same study group were also included for comparison. Associations of superior GS with compatible DRB1 allelic types, serotypes, and grouped DRB1 types were observed. A significantly higher GS rate was found in donor-recipient pairs when HLA DRB1 types were analyzed by group mismatching (P = 0.03) rather than type mismatching (P > 0.34). Because of the numerous allelic types that can be assigned on the basis of nucleotide sequence variations, a larger number of donor-recipient pairs is required to derive statistically significant results.
通过使用序列特异性寡核苷酸对91对肾供体 - 受体进行分析，确定HLA DRB1等位基因类型，并对其进行了30个月的随访。采用Kaplan - Meier方法评估匹配组和不匹配组的移植物存活率（GS）。通过对供体 - 受体对的等位基因类型匹配和不匹配来衡量相容性程度。此外，HLA DR基因型被分为五组，即DR1、DR2、DR4、同时具有DRw52等位基因的组以及DR7、DR9和DR10组。组内的所有类型都被视为匹配。同一研究组的血清型也被纳入比较。观察到较高的移植物存活率与相容的DRB1等位基因类型、血清型和分组的DRB1类型之间的关联。当通过组错配（P = 0.03）而非类型错配（P > 0.34）分析HLA DRB1类型时，供体 - 受体对中的移植物存活率显著更高。由于可以根据核苷酸序列变异确定众多等位基因类型，因此需要更多数量的供体 - 受体对才能得出具有统计学意义的结果。
