对促肾上腺皮质激素体内反应不同的猪的肾上腺皮质促肾上腺皮质激素受体

Adrenocortical ACTH receptors in pigs of differing in vivo response to adrenocorticotropin.

Zhang S H, Hennessy D P, McCauley I, Cranwell P D

School of Agriculture, La Trobe University, Bundoora, Victoria, Australia.

Comp Biochem Physiol Comp Physiol. 1993 Jan;104(1):43-9. doi: 10.1016/0300-9629(93)90006-p.

Abstract

Adrenocortical membrane protein was isolated from the adrenal glands of 12 Large White x Landrace male pigs, six with high adrenocortical response to adrenocorticotropic hormone (ACTH) and six with low response. 2. The peptide (Phe2, Nle4) ACTH was iodinated by the chloramine-T method and served as the radioligand in receptor binding studies. 3. Only one class of ACTH receptor was detected, with Kd = 2.57 +/- 0.35 x 10(9) M and Bmax = 1.59 +/- 0.06 pmol/mg protein in high responders and Kd = 1.68 +/- 0.18 x 10(-9) M and Bmax = 1.17 +/- 0.11 pmol/mg protein in low responders. 4. The difference in the Bmax between high and low responders was significant (P < 0.05), the difference in Kd was not statistically significant.

摘要

从12头大白猪与长白猪杂交的雄性猪的肾上腺中分离出肾上腺皮质膜蛋白，其中6头对促肾上腺皮质激素（ACTH）有高肾上腺皮质反应，6头有低反应。2. 肽（苯丙氨酸2，亮氨酸4）促肾上腺皮质激素通过氯胺-T法进行碘化，并用作受体结合研究中的放射性配体。3. 仅检测到一类促肾上腺皮质激素受体，高反应者的解离常数（Kd）=2.57±0.35×10⁻⁹M，最大结合容量（Bmax）=1.59±0.06 pmol/mg蛋白，低反应者的Kd =1.68±0.18×10⁻⁹M，Bmax =1.17±0.11 pmol/mg蛋白。4. 高反应者与低反应者之间的Bmax差异显著（P<0.05），Kd差异无统计学意义。