Yada Y, Polakowska R R, Okano Y, Nozawa Y
Department of Biochemistry, Gifu University School of Medicine, Japan.
Biochem Biophys Res Commun. 1993 Feb 15;190(3):688-94. doi: 10.1006/bbrc.1993.1104.
Ganglioside GQ1b was found to induce terminal differentiation in normal epidermal keratinocytes (Yada et al. (1991) Biochem. J. 279, 665-670). In GQ1b-stimulated human keratinocytes, the increase in mRNA expression of Type I TGase preceded the increases in the TGase activity. The enhancement of its mRNA expression was inhibited when protein kinase C (PKC) was suppressed by pretreatment with an inhibitor, H-7, or down-regulation by long-term preincubation with phorbol dibutyrate. These results indicate that PKC is involved in the activation of TGase probably via gene expression of TGase mRNA in GQ1b-induced human keratinocyte differentiation. Similar results were also obtained in Ca(2+)-induced differentiation.
神经节苷脂GQ1b被发现可诱导正常表皮角质形成细胞发生终末分化（矢田等人，《生物化学杂志》，1991年，第279卷，665 - 670页）。在GQ1b刺激的人角质形成细胞中，I型转谷氨酰胺酶（TGase）mRNA表达的增加先于TGase活性的增加。当用抑制剂H - 7预处理抑制蛋白激酶C（PKC），或通过与佛波酯二丁酸盐长期预孵育下调PKC时，其mRNA表达的增强受到抑制。这些结果表明，在GQ1b诱导的人角质形成细胞分化过程中，PKC可能通过TGase mRNA的基因表达参与TGase的激活。在钙离子诱导的分化过程中也获得了类似结果。
