人鸟苷素的循环生物活性形式是一种高分子量肽（10.3千道尔顿）。

The circulating bioactive form of human guanylin is a high molecular weight peptide (10.3 kDa).

作者信息

Kuhn M, Raida M, Adermann K, Schulz-Knappe P, Gerzer R, Heim J M, Forssmann W G

机构信息

Niedersächsisches Institut für Peptid-Forschung (IPF), Hannover, Germany.

出版信息

FEBS Lett. 1993 Mar 1;318(2):205-9. doi: 10.1016/0014-5793(93)80022-m.

DOI:10.1016/0014-5793(93)80022-m

PMID:8095028

Abstract

Guanylin is a peptide isolated from rat intestine that stimulates intestinal guanylate cyclase. We describe here the purification of circulating guanylin from human hemofiltrate. By N-terminal protein sequence analysis 47 amino acids were determined. This sequence corresponds to the positions 22 to 68 of the prohormone deduced from the cDNA sequence of human proguanylin. Mass spectral analysis of the circulating peptide showed the molecular weight to be 10,336 Da, which corresponds to the mass calculated from position 22 to the C-terminus of the peptide predicted from the cDNA sequence. Circulating guanylin markedly increased the cyclic GMP content of T84 cells. Our data show that the hormonal form of guanylin is circulating as a 10.3-kDa peptide in human blood.

摘要

鸟苷林是一种从大鼠肠道中分离出来的能刺激肠道鸟苷酸环化酶的肽。我们在此描述了从人血液滤过液中纯化循环鸟苷林的过程。通过N端蛋白质序列分析确定了47个氨基酸。该序列与人前鸟苷林cDNA序列推导的前体激素的第22至68位相对应。对循环肽的质谱分析显示分子量为10336 Da，这与从cDNA序列预测的肽的第22位到C端计算出的质量相符。循环鸟苷林显著增加了T84细胞的环鸟苷酸含量。我们的数据表明，鸟苷林的激素形式在人血液中以10.3 kDa的肽形式循环。