Gorka Z, Adamik P
Institute of Pharmacology, Polish Academy of Sciences, Krakow.
J Pharm Pharmacol. 1993 Feb;45(2):137-8. doi: 10.1111/j.2042-7158.1993.tb03698.x.
The effect of reserpine (2.5 mg kg-1 subcutaneously) and stress (3 h restraint) on food and water consumption and body weight change in the light and dark phases of the diurnal cycle in rats was investigated. Reserpine increased water intake and body weight loss in the light phase (250 and 180% of the control, respectively). In the dark phase the reserpinized rats consumed less food and water (20 and 30% of the control, respectively) and body weight gain was reversed (-250% of the control). After stress, rats consumed more food and water (200 and 500% of the control, respectively) and their body weight loss was diminished (60% of the control) in the light phase. In the dark phase the consumption of food and water in the stressed rats was diminished (80 and 85% of the control, respectively) without any change in body weight gain. The dark/light phase ratio for food consumption, water intake and body weight change (gain/loss) was highly statistically significant in the stressed and reserpine-treated rats. The results indicate that evaluation of behaviour in animal models should be carried out during both phases of the diurnal cycle.
研究了利血平（2.5毫克/千克皮下注射）和应激（3小时束缚）对大鼠昼夜周期明相和暗相中食物和水消耗以及体重变化的影响。利血平增加了明相中的水摄入量和体重减轻（分别为对照组的250%和180%）。在暗相中，注射利血平的大鼠消耗的食物和水较少（分别为对照组的20%和30%），体重增加逆转（为对照组的-250%）。应激后，大鼠在明相中消耗更多的食物和水（分别为对照组的200%和500%），体重减轻减少（为对照组的60%）。在暗相中，应激大鼠的食物和水消耗减少（分别为对照组的80%和85%），体重增加无变化。在应激和利血平处理的大鼠中，食物消耗、水摄入量和体重变化（增加/减少）的暗/明相比具有高度统计学意义。结果表明，动物模型中的行为评估应在昼夜周期的两个阶段进行。
