关于人类牙菌斑的研究。3. 个体间蛋白质成分的差异。

Studies on human dental plaque. 3. Variation of protein constituents among individuals.

作者信息

Holt R L

出版信息

J Oral Pathol. 1975 Sep;4(2):96-100. doi: 10.1111/j.1600-0714.1975.tb01857.x.

DOI:10.1111/j.1600-0714.1975.tb01857.x

Abstract

Separate plaque samples (collected from 13 patients who had experienced caries and various degrees of periodontal disease) were each dispersed in 1 ml of distilled water, homogenized and lyophilized. Each lyophilisate was extracted in 1 ml of buffered saline, concentrated and analyzed. Enzyme activity studies revealed amylase in all plaque samples. Lysozyme was present occasionally. By radial immunodiffusion, IgG and IgA were shown to be in plaque extracts of some patients. Immunofluorescence examination of the sediments of individual plaque samples revealed that IgG and IgA occurred frequently. Polyacrylamide gel electrophoresis patterns of the extracts of plaque from different individuals exhibited marked variability despite some zones of similarity. Peptide maps of the dialysable material of the individual plaque extracts were remarkably similar.

摘要

分别从13例患有龋齿和不同程度牙周疾病的患者中采集菌斑样本，将每个样本分散于1毫升蒸馏水中，匀浆后冻干。将每个冻干物用1毫升缓冲盐水提取，浓缩并进行分析。酶活性研究显示，所有菌斑样本中均存在淀粉酶，溶菌酶偶尔出现。通过放射免疫扩散法发现，部分患者的菌斑提取物中存在IgG和IgA。对单个菌斑样本沉淀物进行免疫荧光检查发现，IgG和IgA频繁出现。尽管不同个体菌斑提取物的聚丙烯酰胺凝胶电泳图谱存在一些相似区域，但仍表现出明显的变异性。单个菌斑提取物可透析物质的肽图非常相似。