Sommerlad B C, Creasey J M
J Microsc. 1975 Apr;103(3):369-76. doi: 10.1111/j.1365-2818.1975.tb03992.x.
The aims in a current study of dermis and scar tissue using the scanning electron microscope are: (1) to maintain the orientation of the specimen, (2) to preserve the in vivo architecture, and (3) to expose a satisfactory surface. A technique which best fulfils these requirements involves glutaraldehyde fixation of a splinted specimen, freeze-fracturing using a modified pair of pliers, freeze-drying again after washing. Consistently satisfactory specimens have been obtained, which provide information about fibre architecture and cell distribution.
