Feng G S, Hui C C, Pawson T
Division of Molecular and Developmental Biology, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada.
Science. 1993 Mar 12;259(5101):1607-11. doi: 10.1126/science.8096088.
A mouse phosphotyrosine phosphatase containing two Src homology 2 (SH2) domains, Syp, was identified. Syp bound to autophosphorylated epidermal growth factor (EGF) and platelet-derived growth factor (PDGF) receptors through its SH2 domains and was rapidly phosphorylated on tyrosine in PDGF- and EGF-stimulated cells. Furthermore, Syp was constitutively phosphorylated on tyrosine in cells transformed by v-src. This mammalian phosphatase is most closely related, especially in its SH2 domains, to the corkscrew (csw) gene product of Drosophila, which is required for signal transduction downstream of the Torso receptor tyrosine kinase. The Syp gene is widely expressed throughout embryonic mouse development and in adult tissues. Thus, Syp may function in mammalian embryonic development and as a common target of both receptor and nonreceptor tyrosine kinases.
一种含有两个Src同源2（SH2）结构域的小鼠磷酸酪氨酸磷酸酶——Syp被鉴定出来。Syp通过其SH2结构域与自身磷酸化的表皮生长因子（EGF）和血小板衍生生长因子（PDGF）受体结合，并在PDGF和EGF刺激的细胞中迅速在酪氨酸上磷酸化。此外，在由v-src转化的细胞中，Syp在酪氨酸上持续磷酸化。这种哺乳动物磷酸酶与果蝇的螺旋（csw）基因产物关系最为密切，尤其是在其SH2结构域方面，而csw基因产物是Torso受体酪氨酸激酶下游信号转导所必需的。Syp基因在整个小鼠胚胎发育过程以及成年组织中广泛表达。因此，Syp可能在哺乳动物胚胎发育中发挥作用，并作为受体和非受体酪氨酸激酶的共同靶点。
