Zalabák V, Babicka J, Chaloupka J
Mikrobiologiia. 1975 Jul-Aug;44(4):720-6.
The amino acid composition of protein was studied with Bacillus megaterium. Most of the essential amino acids are present in sufficient amounts, with the exception of sulphur-containing amino acids and probably tryptophan. The content of methionine is 2 percent. The proteins of the biomass are easily digested by trypsin. The culture of Bac. megaterium grows in a mineral medium containing 5 percent of sucrose or glucose. The yield of dry biomass recalculated for the sugar consumed decreases from 0.6 to 0.3 with an increase of the sugar concentration. The addition of molasses or corn steep increases the yield of the biomass to 15--20 g of dry matter per one litre of the medium. The biomass grown under these conditions contains 8--9 percent of nitrogen, 40 percent of protein, up to 30 percent of poly-beta-hydroxybutyrate in the form of granular inclusions, and 10--14 percent of RNA.
用巨大芽孢杆菌研究了蛋白质的氨基酸组成。除含硫氨基酸和可能的色氨酸外,大多数必需氨基酸含量充足。蛋氨酸含量为2%。生物质中的蛋白质很容易被胰蛋白酶消化。巨大芽孢杆菌在含有5%蔗糖或葡萄糖的矿物培养基中生长。根据消耗的糖重新计算,随着糖浓度的增加,干生物质产量从0.6降至0.3。添加糖蜜或玉米浆可使生物质产量提高到每升培养基15 - 20克干物质。在这些条件下生长的生物质含有8 - 9%的氮、40%的蛋白质、高达30%呈颗粒状内含物形式的聚-β-羟基丁酸酯,以及10 - 14%的RNA。