Brändén H, Lundgren E
Department for Cell and Molecular Biology, University of Umeå, Sweden.
Cell Immunol. 1993 Mar;147(1):64-72. doi: 10.1006/cimm.1993.1048.
Changes in adhesive properties play important regulatory roles in activation and differentiation of B-cells. To better understand the regulation of interactions between B-cells and other cells during the immune response, we have studied surface expression of the adhesion molecules LFA-1 (CD11a/CD18) and ICAM-1 (CD54). Both adhesion molecules were upregulated during B-cell activation. However, upon stimulation with anti-IgM and IL-4, ICAM-1 levels started to increase within 12 hr, while LFA-1 levels did not start to increase until after 36 hr. When B-cells were stimulated with the PKC activator PDB and a calcium ionophore, ICAM-1 levels, but not LFA-1 levels, increased. Only if these activators were removed after around 24 hr of activation and the cells were recultured in fresh medium was there an eightfold induction of LFA-1. Such reculturing in fresh medium led, however, to decreased ICAM-1 levels.
黏附特性的变化在B细胞的激活和分化过程中发挥着重要的调节作用。为了更好地理解免疫反应期间B细胞与其他细胞之间相互作用的调节机制，我们研究了黏附分子LFA-1（CD11a/CD18）和ICAM-1（CD54）的表面表达情况。在B细胞激活过程中，这两种黏附分子均上调。然而，在用抗IgM和IL-4刺激后，ICAM-1水平在12小时内开始升高，而LFA-1水平直到36小时后才开始升高。当用PKC激活剂PDB和钙离子载体刺激B细胞时，ICAM-1水平升高，而LFA-1水平未升高。只有在激活约24小时后去除这些激活剂，并将细胞在新鲜培养基中重新培养，LFA-1才会有八倍的诱导。然而，在新鲜培养基中进行这种重新培养会导致ICAM-1水平降低。
