氟奋乃静氮芥对D2多巴胺受体的不可逆阻断可增加大鼠纹状体中谷氨酸脱羧酶的信使核糖核酸。

Irreversible blockade of D2 dopamine receptors by fluphenazine-N-mustard increases glutamic acid decarboxylase mRNA in rat striatum.

作者信息

Chen J F, Weiss B

机构信息

Department of Pharmacology, Medical College of Pennsylvania, Philadelphia 19129.

出版信息

Neurosci Lett. 1993 Feb 19;150(2):215-8. doi: 10.1016/0304-3940(93)90539-w.

DOI:10.1016/0304-3940(93)90539-w

PMID:8097031

Abstract

The influence of dopaminergic activity on the function of GABAergic neurons in striatum was examined by administering rats the irreversible D2 dopamine receptor antagonist, fluphenazine-N-mustard (FNM), and determining the level of glutamic acid decarboxylase (GAD) mRNA in striatum. Rats were given either an acute single injection or chronic daily injections of FNM (20 mumol/kg, i.p.) for 6 days. The level of GAD mRNA in striatum was determined by in situ hybridization histochemistry. The results showed that acute treatment with FNM failed to significantly change striatal GAD mRNA. However, chronic FNM treatment significantly increased in the level of striatal GAD mRNA. These results demonstrate that irreversible blockade of D2 dopamine receptors increases the expression of GAD mRNA in rat striatum.

摘要

通过给大鼠注射不可逆的D2多巴胺受体拮抗剂氟奋乃静氮芥（FNM），并测定纹状体中谷氨酸脱羧酶（GAD）mRNA的水平，研究了多巴胺能活性对纹状体中γ-氨基丁酸能（GABAergic）神经元功能的影响。给大鼠单次急性注射或连续6天每天注射FNM（20 μmol/kg，腹腔注射）。通过原位杂交组织化学法测定纹状体中GAD mRNA的水平。结果表明，FNM急性处理未能显著改变纹状体GAD mRNA水平。然而，FNM慢性处理显著提高了纹状体GAD mRNA水平。这些结果表明，不可逆地阻断D2多巴胺受体会增加大鼠纹状体中GAD mRNA的表达。