Gordon L, Wharton J, Gaer J A, Inglis G C, Taylor K M, Polak J M
Royal Postgraduate Medical School, London, United Kingdom.
Cardiovasc Res. 1993 Feb;27(2):318-26. doi: 10.1093/cvr/27.2.318.
The aim was to determine the relative distribution and possible origins of peptide containing nerves in the bovine heart before and after functional extrinsic denervation established by cryosurgery.
A quantitative immunohistochemical technique was used.
In the intact heart, myocardial nerve fibres and fascicles displaying immunoreactivity for the general neural marker protein gene product 9.5 had an atrial to ventricular gradient in density. The right atrium was the most densely innervated region and a major proportion of the total myocardial innervation, visualised by protein gene product 9.5 immunoreactive nerves, showed neuropeptide tyrosine (45%) and tyrosine hydroxylase (20%) immunofluorescence staining, while nerves immunoreactive for vasoactive intestinal polypeptide and calcitonin gene related peptide formed relatively minor subpopulations (representing less than 2% and 0.5%, respectively, of the total fluorescent myocardial innervation). Following cryoablation there was a significant reduction in the percentage fluorescent area of protein gene product 9.5 immunoreactive nerves throughout the heart, of greater than 90% of the control values. There were highly significant reductions in the percentage fluorescent area of nerves showing immunoreactivity to neuropeptide tyrosine, tyrosine hydroxylase, and calcitonin gene related peptide, to 1.01, 0.92, and 0.05%, respectively, of the intact myocardial innervation. The distribution of vasoactive intestinal polypeptide immunoreactive nerves was more variable and displayed an equivocal response to cardiac cryoablation.
The majority of nerves showing immunoreactivity to neuropeptide tyrosine, tyrosine hydroxylase, and calcitonin gene related peptide are of extrinsic origin, while vasoactive intestinal polypeptide immunoreactive nerves may have intrinsic as well as extrinsic origins. The distribution and apparent origins of immunohistochemically defined nerves in the bovine heart are similar to those observed in the human heart which suggests that the calf may be an appropriate model for comparative studies.
本研究旨在确定在通过冷冻手术建立功能性外在去神经支配前后，牛心脏中含肽神经的相对分布及可能来源。
采用定量免疫组织化学技术。
在完整心脏中，对通用神经标志物蛋白基因产物9.5呈免疫反应性的心肌神经纤维和神经束，其密度存在心房到心室的梯度。右心房是神经支配最密集的区域，通过蛋白基因产物9.5免疫反应性神经显示的心肌总神经支配的很大一部分，呈现神经肽Y（45%）和酪氨酸羟化酶（20%）免疫荧光染色，而对血管活性肠肽和降钙素基因相关肽呈免疫反应性的神经形成相对较小的亚群（分别占荧光心肌总神经支配的不到2%和0.5%）。冷冻消融后，整个心脏中蛋白基因产物9.5免疫反应性神经的荧光面积百分比显著降低，大于对照值的90%。对神经肽Y、酪氨酸羟化酶和降钙素基因相关肽呈免疫反应性的神经的荧光面积百分比显著降低，分别降至完整心肌神经支配的1.01%、0.92%和0.05%。血管活性肠肽免疫反应性神经的分布更具变异性，对心脏冷冻消融的反应不明确。
对神经肽Y、酪氨酸羟化酶和降钙素基因相关肽呈免疫反应性的大多数神经为外在起源，而血管活性肠肽免疫反应性神经可能有内在和外在起源。牛心脏中免疫组织化学定义的神经的分布和明显起源与人类心脏中观察到的相似，这表明小牛可能是进行比较研究的合适模型。
