家猪体内羟基化维生素D代谢产物[25-羟基维生素D3和1,25-二羟基维生素D3]的分布：1,25-二羟基维生素D3是否储存在血液循环外的证据？

Distribution of hydroxylated vitamin D metabolites [25OHD3 and 1,25(OH)2D3] in domestic pigs: evidence that 1,25(OH)2D3 is stored outside the blood circulation?

Rungby J, Mortensen L, Jakobsen K, Brock A, Mosekilde L

University Department of Endocrinology and Metabolism, Aarhus County Hospital, Denmark.

Comp Biochem Physiol Comp Physiol. 1993 Mar;104(3):483-4. doi: 10.1016/0300-9629(93)90451-9.

Abstract

The distribution of 25-hydroxycholecalciferol (25OHD3) and 1,25-dihydroxycholecalciferol [1,25(OH)2D3] in various organs from domestic pigs was examined by HPLC. 2. Plasma levels of both metabolites corresponded to those found in healthy human subjects. 3. Tissue concentrations of 25OHD3 in fat, kidney, liver, and intestinal mucosa were low (< 1/3 of plasma levels), whereas tissue concentrations of 1,25(OH)2D3 exceeded plasma levels by factors 3-7, adipose tissue concentrations being the highest. 4. Substantial amounts of activated vitamin D are stored outside the blood-streams and may actively participate in vitamin D and calcium homeostasis.

摘要

采用高效液相色谱法检测了家猪各器官中25-羟基胆钙化醇（25OHD3）和1,25-二羟基胆钙化醇[1,25(OH)2D3]的分布情况。2. 两种代谢物的血浆水平与健康人类受试者中的水平相当。3. 脂肪、肾脏、肝脏和肠黏膜中25OHD3的组织浓度较低（<血浆水平的1/3），而1,25(OH)2D3的组织浓度超过血浆水平3-7倍，脂肪组织中的浓度最高。4. 大量活化维生素D储存在血流之外，可能积极参与维生素D和钙的稳态调节。