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体外猿猴病毒40 DNA复制过程中引物-DNA的形成

Primer-DNA formation during simian virus 40 DNA replication in vitro.

作者信息

Denis D, Bullock P A

机构信息

Department of Biochemistry, Tufts University School of Medicine, Boston, Massachusetts 02111.

出版信息

Mol Cell Biol. 1993 May;13(5):2882-90. doi: 10.1128/mcb.13.5.2882-2890.1993.

DOI:10.1128/mcb.13.5.2882-2890.1993
PMID:8097277
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC359681/
Abstract

Studies of simian virus 40 (SV40) DNA replication in vitro have identified a small (approximately 30-nucleotide) RNA-DNA hybrid species termed primer-DNA. Initial experiments indicated that T antigen and the polymerase alpha-primase complex are required to form primer-DNA. Proliferating cell nuclear antigen, and presumably proliferating cell nuclear antigen-dependent polymerases, is not needed to form this species. Herein, we present an investigation of the stages at which primer-DNA functions during SV40 DNA replication in vitro. Hybridization studies indicate that primer-DNA is initially formed in the origin region and is subsequently synthesized in regions distal to the origin. At all time points, primer-DNA is synthesized from templates for lagging-strand DNA replication. These studies indicate that primer-DNA functions during both initiation and elongation stages of SV40 DNA synthesis. Results of additional experiments suggesting a precursor-product relationship between formation of primer-DNA and Okazaki fragments are presented.

摘要

对猿猴病毒40（SV40）体外DNA复制的研究鉴定出一种小的（约30个核苷酸）RNA-DNA杂交分子，称为引物-DNA。最初的实验表明，T抗原和聚合酶α-引发酶复合物是形成引物-DNA所必需的。增殖细胞核抗原以及推测的依赖增殖细胞核抗原的聚合酶在形成该分子时并非必需。在此，我们对引物-DNA在SV40体外DNA复制过程中发挥作用的阶段进行了研究。杂交研究表明，引物-DNA最初在起始区域形成，随后在起始点远端的区域合成。在所有时间点，引物-DNA都是从滞后链DNA复制的模板合成的。这些研究表明，引物-DNA在SV40 DNA合成的起始和延伸阶段均发挥作用。还展示了其他实验结果，这些结果表明引物-DNA的形成与冈崎片段之间存在前体-产物关系。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1277/359681/22dc9f27ccda/molcellb00017-0262-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1277/359681/a98904d53cfc/molcellb00017-0258-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1277/359681/d91e280ca1ca/molcellb00017-0258-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1277/359681/4d989889e7c8/molcellb00017-0260-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1277/359681/615f5f509294/molcellb00017-0261-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1277/359681/22dc9f27ccda/molcellb00017-0262-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1277/359681/a98904d53cfc/molcellb00017-0258-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1277/359681/d91e280ca1ca/molcellb00017-0258-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1277/359681/4d989889e7c8/molcellb00017-0260-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1277/359681/615f5f509294/molcellb00017-0261-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1277/359681/22dc9f27ccda/molcellb00017-0262-a.jpg

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1
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本文引用的文献

1
DNA polymerases delta and epsilon are required for chromosomal replication in Saccharomyces cerevisiae.DNA聚合酶δ和ε是酿酒酵母染色体复制所必需的。
Mol Cell Biol. 1993 Jan;13(1):496-505. doi: 10.1128/mcb.13.1.496-505.1993.
2
Elongation of RNA-primed DNA templates by DNA polymerase alpha from Drosophila melanogaster embryos.黑腹果蝇胚胎的DNA聚合酶α对RNA引发的DNA模板的延伸作用。
J Biol Chem. 1981 Aug 10;256(15):8202-7.
3
Further studies on calf thymus DNA polymerase delta purified to homogeneity by a new procedure.通过一种新方法对纯化至同质的小牛胸腺DNA聚合酶δ进行的进一步研究。
Vts1，一种结构特异性 RNA 结合蛋白，参与酵母中 Okazaki 片段的加工。
Nucleic Acids Res. 2010 Mar;38(5):1583-95. doi: 10.1093/nar/gkp1135. Epub 2009 Dec 9.
4
What is your assay for sister-chromatid cohesion?你用于检测姐妹染色单体黏连的分析方法是什么？
EMBO J. 2007 Nov 14;26(22):4609-18. doi: 10.1038/sj.emboj.7601898. Epub 2007 Oct 25.
5
Interactions between telomerase and primase physically link the telomere and chromosome replication machinery.端粒酶与引发酶之间的相互作用将端粒与染色体复制机制物理连接起来。
Mol Cell Biol. 2002 Aug;22(16):5859-68. doi: 10.1128/MCB.22.16.5859-5868.2002.
6
In the simian virus 40 in vitro replication system, start site selection by the polymerase alpha-primase complex is not significantly altered by changes in the concentration of ribonucleotides.在猿猴病毒40体外复制系统中，核糖核苷酸浓度的变化不会显著改变聚合酶α-引发酶复合物对起始位点的选择。
J Virol. 2001 Jul;75(14):6392-401. doi: 10.1128/JVI.75.14.6392-6401.2001.
7
The replication protein A binding site in simian virus 40 (SV40) T antigen and its role in the initial steps of SV40 DNA replication.猴病毒40（SV40）T抗原中的复制蛋白A结合位点及其在SV40 DNA复制起始步骤中的作用。
J Virol. 1998 Dec;72(12):9771-81. doi: 10.1128/JVI.72.12.9771-9781.1998.
8
The N-terminal side of the origin-binding domain of simian virus 40 large T antigen is involved in A/T untwisting.猿猴病毒40大T抗原的起始结合结构域的N端参与A/T解旋。
J Virol. 1997 Nov;71(11):8743-9. doi: 10.1128/JVI.71.11.8743-8749.1997.
9
Species-specific replication of simian virus 40 DNA in vitro requires the p180 subunit of human DNA polymerase alpha-primase.猿猴病毒40 DNA在体外的种属特异性复制需要人DNA聚合酶α-引发酶的p180亚基。
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10
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4
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5
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6
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7
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9
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Mol Cell Biol. 1986 Nov;6(11):4077-87. doi: 10.1128/mcb.6.11.4077-4087.1986.
10
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EMBO J. 1986 Aug;5(8):1939-44. doi: 10.1002/j.1460-2075.1986.tb04447.x.