Kvesitadze G I, Kokonashvili G N, Sadunishvili T A
Prikl Biokhim Mikrobiol. 1993 Jan-Feb;29(1):131-7.
The activity of enzymes of nitrogen and energy metabolisms from dogfish liver and a commercial preparation Catrex manufactured in the Scientific-Industrial Association "Adaptogen" (Tbilisi) was studied. The liver homogenate contains active glutamate dehydrogenase (GD), malate dehydrogenase (MD) and lactate dehydrogenase (LD) catalysing in vitro the reaction in both directions, as well as active glutamine synthetase, aspartate transaminase and alanine transaminase. These enzymes are also present in Catrex, but their activities are less. After 10-day storage of the liver homogenate and the Catrex preparation the enzymes slightly inactivated. Two isozymes of MD and four isozymes of LD were detected in the liver homogenate by polyacrylamide gel electrophoresis. In Catrex the two MD isozymes and only three LD isozymes were found.
对角鲨肝脏中氮和能量代谢相关酶的活性以及科学工业协会“适应原”(第比利斯)生产的一种商品制剂Catrex进行了研究。肝脏匀浆含有在体外催化双向反应的活性谷氨酸脱氢酶(GD)、苹果酸脱氢酶(MD)和乳酸脱氢酶(LD),以及活性谷氨酰胺合成酶、天冬氨酸转氨酶和丙氨酸转氨酶。这些酶也存在于Catrex中,但它们的活性较低。肝脏匀浆和Catrex制剂储存10天后,酶略有失活。通过聚丙烯酰胺凝胶电泳在肝脏匀浆中检测到两种MD同工酶和四种LD同工酶。在Catrex中仅发现两种MD同工酶和三种LD同工酶。