Wiedermann C J, Reinisch N, Niedermühlbichler M, Braunsteiner H
Department of Internal Medicine, University of Innsbruck, Austria.
Naunyn Schmiedebergs Arch Pharmacol. 1993 Mar;347(3):336-41. doi: 10.1007/BF00167454.
Growth hormone, prolactin and somatostatin are polypeptide hormones of the neuroendocrine and peripheral nervous systems. In vitro, these have opposing effects on cells of the immune system. We compared the effects of these peptides on activation of neutrophils using a recombinant preparation of human growth hormone, human prolactin and octreotide, a long acting analog of somatostatin. In the absence of growth hormone, octreotide did not affect either neutrophil locomotion or respiratory burst. Octreotide, however, significantly antagonized growth hormone-induced activation of neutrophils for enhanced respiratory burst as well as growth hormone-induced inhibition of stimulated migration. As the effect of growth hormone on neutrophils is mediated by the prolactin receptor, its inhibition by octreotide was also tested using prolactin as priming agent. Data indicate comparable effects of octreotide on priming of neutrophils by prolactin. The effect of octreotide was dose-dependent and appeared to be selective, as activation of neutrophil respiration burst by gamma-interferon, and inhibition of stimulated migration by tumor necrosis factor-alpha were unaffected by octreotide. The present study suggests that octreotide may act on neutrophils directly by antagonizing growth hormone or prolactin at the cellular level.
生长激素、催乳素和生长抑素是神经内分泌系统和外周神经系统的多肽激素。在体外，这些激素对免疫系统细胞具有相反的作用。我们使用重组的人生长激素、人催乳素和生长抑素的长效类似物奥曲肽，比较了这些肽对中性粒细胞激活的影响。在没有生长激素的情况下，奥曲肽既不影响中性粒细胞的运动，也不影响其呼吸爆发。然而，奥曲肽显著拮抗生长激素诱导的中性粒细胞激活，从而增强呼吸爆发以及生长激素诱导的刺激迁移抑制。由于生长激素对中性粒细胞的作用是由催乳素受体介导的，因此也使用催乳素作为启动剂测试了奥曲肽对其的抑制作用。数据表明奥曲肽对催乳素启动中性粒细胞具有类似的作用。奥曲肽的作用是剂量依赖性的，并且似乎具有选择性，因为γ干扰素激活中性粒细胞呼吸爆发以及肿瘤坏死因子-α抑制刺激迁移均不受奥曲肽影响。本研究表明，奥曲肽可能通过在细胞水平拮抗生长激素或催乳素而直接作用于中性粒细胞。
