Dirie M F, Otte M J, Thatthi R, Gardiner P R
International Laboratory for Research on Animal Diseases (ILRAD), Nairobi, Kenya.
Parasitology. 1993 Jan;106 ( Pt 1):21-9. doi: 10.1017/s0031182000074771.
The characterization of four Trypanosoma vivax isolates from Colombia in South America showed that although minor phenotypic differences existed between them, these parasites are antigenically related and belong to a single serodeme. Characterization by isoenzyme assay, karyotyping and DNA probe analysis, showed the Colombian isolates to be more similar to the West African than to Kenyan T. vivax. There was, however, little serological cross-reactivity between South American and African groups of T. vivax. Although the T. vivax isolates from Colombia were pathogenic for dairy calves which showed the typical sign of progressive emaciation, these parasites failed to infect mice or tsetse and could not be cultivated as bloodstream forms in vitro. This study represents initial attempts to establish the phenotypic and serological diversity amongst T. vivax isolates from South America.
对来自南美洲哥伦比亚的四株间日锥虫分离株的特性分析表明，尽管它们之间存在微小的表型差异，但这些寄生虫在抗原方面具有相关性，属于单一血清型。通过同工酶分析、核型分析和DNA探针分析进行的特性鉴定表明，哥伦比亚分离株与西非的间日锥虫比与肯尼亚的间日锥虫更为相似。然而，南美洲和非洲的间日锥虫群体之间几乎没有血清学交叉反应。尽管来自哥伦比亚的间日锥虫分离株对表现出典型渐进性消瘦症状的乳牛犊具有致病性，但这些寄生虫未能感染小鼠或采采蝇，也无法在体外培养成血流形式。这项研究是建立南美洲间日锥虫分离株表型和血清学多样性的初步尝试。
