Bièche I, Champème M H, Matifas F, Cropp C S, Callahan R, Lidereau R
Laboratory of Oncovirology, Centre René Huguenin, St-Cloud, France.
Cancer Res. 1993 May 1;53(9):1990-4.
Alteration of chromosome 1 is the most consistent cytogenetic abnormality found in human breast carcinoma. Cytogenetic studies have shown independent alterations on the two arms of chromosome 1, increased copy number of the long arm and loss of the short arm of chromosome 1. These deletions are thought to coincide with the location of tumor suppressor gene(s). We carried out deletion analysis of the 1p region by using restriction fragment length polymorphism markers mapping to the long (six markers) and short arm (22 markers). Thirty-five of the 74 (47.3%) human breast tumors tested showed somatic loss of heterozygosity at one or more loci on the short arm. Two commonly deleted regions, 1p13-p21 and 1p32-pter, were identified. The latter region is frequently involved in other types of tumors, suggesting that it harbors a common tumor suppressor gene. Our findings suggest that two tumor suppressor genes involved in the development of human breast carcinoma may occur on the short arm of the chromosome 1.
1号染色体的改变是在人类乳腺癌中发现的最常见的细胞遗传学异常。细胞遗传学研究表明，1号染色体的两条臂上存在独立的改变，即1号染色体长臂的拷贝数增加和短臂缺失。这些缺失被认为与肿瘤抑制基因的位置一致。我们通过使用定位在长臂（六个标记）和短臂（22个标记）上的限制性片段长度多态性标记，对1p区域进行了缺失分析。在检测的74个人类乳腺肿瘤中，有35个（47.3%）在短臂上的一个或多个位点出现了杂合性体细胞缺失。确定了两个常见的缺失区域，1p13-p21和1p32-pter。后一个区域经常涉及其他类型的肿瘤，这表明它含有一个常见的肿瘤抑制基因。我们的研究结果表明，参与人类乳腺癌发生的两个肿瘤抑制基因可能位于1号染色体的短臂上。
