Department of Medical Biology, Ege University Medical Faculty, Bornova, 35100, Izmir, Turkey,
Ir J Med Sci. 2014 Jun;183(2):259-64. doi: 10.1007/s11845-013-1001-3. Epub 2013 Aug 11.
The Runx family proteins, including RUNX3, are tissue-restricted transcription factors and play role in neuronal development and tumorigenesis. RUNX3 has an important role in glioblastoma (GBM) tumorigenesis because of its promoter hypermethylation.
We aimed to evaluate the methylation-mediated expression regulation of RUNX3 gene in brain tumors.
Cases of meningiomas WHO grade III (3), anaplastic astrocytomas (3), diffuse astrocytoma (3), and GBM (12) were recruited into this study. Real-time quantitative PCR was performed for analyses of DNA promoter methylation and analyses of methylation-mediated expression status of RUNX3 gene was performed by real-time quantitative RT-PCR.
There was no significant difference between methylated and unmethylated quantitative ratio of RUNX3 gene promoter region and also no significant difference in relative ratio of RUNX3 gene expression in brain tumor groups. Methylated and unmethylated ratio in anaplastic astrocytoma, diffuse astrocytoma, GBM, meningioma (WHO grade III) and in all groups were; 1.44, 1.09, 1.51, 1.52 and 1.43, respectively. One allele was found methylated necessarily. No methylation was detected in one case of GBM group and one case of anaplastic astrocytoma group. There was no unmethylated promoter in one of the GBM cases. There were significant differences between relative ratio of RUNX3 gene expression and methylated/unmethylated ratio rates for all cases (p = 0.001) and GBM groups (p = 0.041).
This study overemphasized the RUNX3 gene importance in brain tumors, due to the existence of at least one methylated allele.
Runx 家族蛋白，包括 RUNX3，是组织受限的转录因子，在神经元发育和肿瘤发生中发挥作用。由于启动子超甲基化，RUNX3 在胶质母细胞瘤 (GBM) 肿瘤发生中具有重要作用。
我们旨在评估 RUNX3 基因在脑肿瘤中的甲基化介导的表达调控。
本研究纳入了 III 级脑膜瘤（3 例）、间变性星形细胞瘤（3 例）、弥漫性星形细胞瘤（3 例）和 GBM（12 例）患者。采用实时定量 PCR 分析 DNA 启动子甲基化，采用实时定量 RT-PCR 分析 RUNX3 基因的甲基化介导的表达状态。
RUNX3 基因启动子区域的甲基化和未甲基化定量比之间没有显著差异，脑肿瘤组中 RUNX3 基因表达的相对比值也没有显著差异。间变性星形细胞瘤、弥漫性星形细胞瘤、GBM、脑膜瘤（WHO 分级 III）和所有组中甲基化和未甲基化的比率分别为 1.44、1.09、1.51、1.52 和 1.43。一个等位基因必然发生甲基化。GBM 组和间变性星形细胞瘤组各有 1 例未检测到甲基化。GBM 组中有 1 例无未甲基化启动子。所有病例（p=0.001）和 GBM 组（p=0.041）RUNX3 基因表达的相对比值与甲基化/未甲基化比率之间存在显著差异。
本研究强调了 RUNX3 基因在脑肿瘤中的重要性，因为至少存在一个甲基化等位基因。
