Choi H K, Won L, Heller A
Department of Pharmacological and Physiological Sciences, University of Chicago, IL 60637.
J Neurosci Methods. 1993 Mar;46(3):233-44. doi: 10.1016/0165-0270(93)90072-y.
The use of three-dimensional (3-D) reaggregate cultures of mesencephalic fetal dopaminergic neurons in co-culture with their target cells of the corpus striatum (CS) has permitted examination of the development and survival of such neurons for periods of up to 1 year. Dopaminergic neurons grown in dialyzed serum in reaggregate cultures show an increase in neurotransmitter level over the first 2 months in culture, and these levels of dopamine are maintained or increased over the next 4-12 months. While there is an apparent decrease in the numbers of dopaminergic neurons by 1 year in culture, many dopaminergic neurons survive and can be visualized at the light and electron microscopic level by tyrosine hydroxylase immunocytochemistry and their processes assessed by histofluorescent techniques. The survival of dopaminergic neurons in an organized culture system in which they demonstrate a normal developmental pattern and establish synaptic contact with appropriate target cells provides an approach to the experimental examination of a substantial portion of the life history of these neurons.
将中脑胎儿多巴胺能神经元的三维（3-D）重聚体培养物与纹状体（CS）的靶细胞共培养，使得对这类神经元长达1年的发育和存活情况进行研究成为可能。在重聚体培养物中，在透析血清中生长的多巴胺能神经元在培养的头2个月神经递质水平会升高，并且在接下来的4 - 12个月中这些多巴胺水平会维持或升高。虽然培养1年后多巴胺能神经元数量明显减少，但许多多巴胺能神经元存活下来，并且通过酪氨酸羟化酶免疫细胞化学在光镜和电镜水平上可以观察到它们，其突起可通过组织荧光技术进行评估。多巴胺能神经元在有组织的培养系统中存活，在该系统中它们表现出正常的发育模式并与合适的靶细胞建立突触联系，这为实验研究这些神经元生命历程的很大一部分提供了一种方法。
