Dabo S M, Mitchell E D, Melcher U
Biochemistry and Molecular Biology Department, Oklahoma State University, Stillwater 74078-0454.
Anal Biochem. 1993 Apr;210(1):34-8. doi: 10.1006/abio.1993.1146.
Cotton leaves contain high levels of polyphenolic compounds that irreversibly interact with proteins and nucleic acids during DNA isolation. A procedure to isolate nuclear DNA from cotton (Gossypium hirsutum L.) has been developed. The method is based on the rapid initial isolation of nuclei in a glucose medium designed to stabilize nuclear structure and composition while preventing covalent interactions with polyphenolics. The resulting DNA is high in yield and purity and is suitable for Southern-blot hybridization analysis and restriction fragment length polymorphism analysis.
棉花叶片含有高水平的多酚化合物，在DNA分离过程中会与蛋白质和核酸发生不可逆的相互作用。现已开发出一种从棉花（陆地棉）中分离核DNA的方法。该方法基于在葡萄糖培养基中快速初步分离细胞核，该培养基旨在稳定核结构和组成，同时防止与多酚发生共价相互作用。所得到的DNA产量高、纯度高，适用于Southern杂交分析和限制性片段长度多态性分析。
