适用于重组DNA技术的高分子量植物核DNA的分离。
Isolation of high molecular weight plant nuclear DNA suitable for use in recombinant DNA technology.
作者信息
Kiss T, Solymosy F
机构信息
Institute of Plant Physiology, Hungarian Academy of Sciences, Szeged.
出版信息
Acta Biochim Biophys Hung. 1987;22(1):1-5.
Nuclei isolated from leaf cells of broad bean (Vicia faba L) by a newly developed method based on the use of citric acid in the isolation medium and flotation on a Percoll cushion yielded high molecular weight plant nuclear DNA which was suitable for (i) analysis by restriction endonucleases, (ii) molecular cloning and (iii) genomic blot hybridization. Starting from nuclear preparations obtained by this method, U2 small nuclear RNA-specific DNA sequences were detected in Vicia faba L. This is the first report on the demonstration of small nuclear RNA-specific DNA sequences in plant material.
通过一种新开发的方法从蚕豆（Vicia faba L）叶细胞中分离细胞核，该方法基于在分离培养基中使用柠檬酸并在Percoll垫层上进行浮选，得到了高分子量的植物核DNA，其适用于：（i）用限制性内切酶进行分析；（ii）分子克隆；（iii）基因组印迹杂交。从通过该方法获得的核制剂开始，在蚕豆中检测到了U2小核RNA特异性DNA序列。这是关于在植物材料中证明小核RNA特异性DNA序列的首次报道。
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