通过聚合酶链反应方法在人膀胱冲洗液癌细胞中检测到基因扩增。
Gene amplification detected in carcinoma cells from human urinary bladder washings by the polymerase chain reaction method.
作者信息
Lönn U, Lönn S, Nylén U, Friberg S, Stenkvist B
机构信息
Radiumhemmet, Karolinska Hospital, Stockholm, Sweden.
出版信息
Cancer. 1993 Jun 1;71(11):3605-10. doi: 10.1002/1097-0142(19930601)71:11<3605::aid-cncr2820711123>3.0.co;2-l.
BACKGROUND
Urinary bladder carcinoma often is diagnosed from malignant cells in bladder washings obtained at cystoscopic examination. In some cases, there are difficulties distinguishing between cytologic Grades 1 and 2. Detection of genetic alterations in combination with morphologic analysis may facilitate the diagnosis.
METHODS
The presence of amplified c-erb-B2 and epidermal growth factor receptor (EGF-R) was examined in tumor cells present in bladder washings. The gene copy number was determined with the polymerase chain reaction (PCR).
RESULTS
The authors detected amplified c-erb-B2 and EGF-R in cancer cells of cytologic Grade 2 and 3 tumors. They did not detect amplification in cytologic Grade 1 tumor cells or in cells from bladders, without known malignant neoplasms.
CONCLUSIONS
Examination of genetic alterations, in combination with morphologic analysis, facilitates the diagnosis of carcinoma.
背景
膀胱癌常通过膀胱镜检查时获取的膀胱冲洗液中的恶性细胞来诊断。在某些情况下，区分细胞病理学1级和2级存在困难。结合形态学分析检测基因改变可能有助于诊断。
方法
检测膀胱冲洗液中肿瘤细胞中扩增的c-erb-B2和表皮生长因子受体（EGF-R）的存在情况。用聚合酶链反应（PCR）测定基因拷贝数。
结果
作者在细胞病理学2级和3级肿瘤的癌细胞中检测到扩增的c-erb-B2和EGF-R。在细胞病理学1级肿瘤细胞或来自无已知恶性肿瘤的膀胱的细胞中未检测到扩增。
结论
结合形态学分析检查基因改变有助于癌的诊断。
Zotero 插件