Dozier C, Carrière C, Grévin D, Martin P, Quatannens B, Stéhelin D, Saule S
Centre National de la Recherche Scientifique, URA 1160, Institut Pasteur de Lille, France.
Cell Growth Differ. 1993 Apr;4(4):281-9.
After differential screening of a complementary DNA library constructed from quail neuroretina cells (QNR) infected with the v-myc-containing avian retrovirus MC29, we have isolated a complementary DNA clone which identifies a mRNA essentially expressed in the neuronal layer of the retina. This complementary DNA encodes a protein containing paired box and homeobox domains. This gene, called Pax-QNR, is homologous to the murine Pax-6 and human AN genes, which are mutated in the autosomal dominant mutation small eye (Sey) of the mouse and aniridia in humans. Here, we report the genomic exon-intron organization, as well as the existence of alternative splicing events taking place at both the 5' end and the middle part of the gene. A Pax-QNR clone translated in reticulocyte lysate directed the synthesis of a 46 kilodalton protein able to bind specifically to the e5 sequence present upstream of the Drosophila even-skipped gene and target of the Drosophila paired protein. The Pax-QNR paired and homeobox domains individually expressed in bacteria are both able to recognize the e5 sequence.
在用含v-myc的禽逆转录病毒MC29感染的鹌鹑神经视网膜细胞（QNR）构建的互补DNA文库进行差异筛选后，我们分离出一个互补DNA克隆，该克隆鉴定出一种主要在视网膜神经元层表达的mRNA。这个互补DNA编码一种含有成对盒和同源盒结构域的蛋白质。这个基因，称为Pax-QNR，与小鼠的Pax-6和人类的AN基因同源，它们在小鼠的常染色体显性突变小眼（Sey）和人类的无虹膜症中发生了突变。在这里，我们报告了该基因的基因组外显子-内含子组织，以及在基因的5'端和中部发生的可变剪接事件。在网织红细胞裂解物中翻译的Pax-QNR克隆指导合成一种46千道尔顿的蛋白质，该蛋白质能够特异性结合果蝇even-skipped基因上游存在的e5序列以及果蝇成对蛋白的靶标。在细菌中单独表达的Pax-QNR成对结构域和同源盒结构域都能够识别e5序列。
