Wall D M, Sparrow R, Hu X F, Nadalin G, Zalcberg J R, Marschner I C, Van der Weyden M, Parkin J D
Department of Haematology, Heidelberg Repatriation Hospital.
Eur J Cancer. 1993;29A(7):1024-7. doi: 10.1016/s0959-8049(05)80216-3.
A rapid and simple functional assay for P-glycoprotein (Pgp) using flow cytometry to measure the accumulation of the flurophore fluo-3 has been applied to samples from patients with B-cell chronic lymphocytic leukaemia (B-CLL). Peripheral blood lymphocytes from 37 patients with B-CLL were studied for Pgp. Pgp expression, using MRK-16, a monoclonal antibody recognising an external surface epitope of Pgp, was detected in 92% of patients with B-CLL. The functional assays for Pgp expression were positive in 78 and 59% of patients using the fluo-3 and doxorubicin (dox) assays, respectively. When compared with the MRK-16 assay, the fluo-3 assay had a sensitivity of 82% compared to a sensitivity of 56% for the dox assay (P = 0.004). The specificity of the fluo-3 and dox assays could not be evaluated because of the low number of MRK-16 negative CLL cells.
一种使用流式细胞术测量荧光团Fluo-3积累的快速简单的P-糖蛋白（Pgp）功能检测方法已应用于B细胞慢性淋巴细胞白血病（B-CLL）患者的样本。对37例B-CLL患者的外周血淋巴细胞进行了Pgp研究。使用识别Pgp外表面表位的单克隆抗体MRK-16检测到92%的B-CLL患者有Pgp表达。使用Fluo-3和阿霉素（dox）检测方法，Pgp表达功能检测分别在78%和59%的患者中呈阳性。与MRK-16检测相比，Fluo-3检测的灵敏度为82%，而dox检测的灵敏度为56%（P = 0.004）。由于MRK-16阴性CLL细胞数量较少，无法评估Fluo-3和dox检测的特异性。
