Manavathu E K
Department of Medicine, Wayne State University School of Medicine, Detroit, Michigan 48201.
Indian J Exp Biol. 1993 Mar;31(3):206-9.
The glycolytic enzyme phosphoglycerate kinase (PGK) is highly conserved throughout the evolutionary ladder. Using a previously cloned Saccharomyces cerevisiae PGK gene as hybridization probe, chromosomal localization and strain-specific variation of PGK gene in C. albicans have been examined. Separation of chromosome-size DNA fragments by pulse field gel electrophoresis followed by DNA- DNA hybridization analysis revealed that the PGK is encoded by a single copy gene located on the large chromosome of the commonly used laboratory strain ATCC 32354, as well as of several clinical isolates of C. albicans. Restriction fragment length analysis indicated that the PGK gene locus is highly polymorphic, and this strain-specific variation could be exploited to study intra-specific relatedness of clinical isolates.
糖酵解酶磷酸甘油酸激酶（PGK）在整个进化过程中高度保守。利用先前克隆的酿酒酵母PGK基因作为杂交探针，对白色念珠菌中PGK基因的染色体定位和菌株特异性变异进行了研究。通过脉冲场凝胶电泳分离染色体大小的DNA片段，随后进行DNA-DNA杂交分析，结果显示PGK由位于常用实验室菌株ATCC 32354以及几种白色念珠菌临床分离株的大染色体上的单拷贝基因编码。限制性片段长度分析表明，PGK基因位点具有高度多态性，这种菌株特异性变异可用于研究临床分离株的种内相关性。
