Marmont A M, Santini G, Bacigalupo A, Damasio E E, Giordano D
Acta Haematol. 1975;54(3):129-42. doi: 10.1159/000208064.
When leukaemic lymphoblasts from acute lymphatic leukaemia were reacted with animal anti-human lymphocyte globulin (AHLG), and subsequently with the appropriate, fluorescein-isothiocyanate-conjugated antibodies, a brilliant membrane fluorescence was observed, which persisted up to 5 log titres, breaking from rings into spots at higher dilutions. The best results were obtained with fresh suspensions and incident illumination. Cross-reactions with mature and immature myeloid cells with the unabsorbed antisera could be abolished by repeated preabsorbtion of AHLG with packed leukocyte preparations from chronic and acute myeloid leukaemias. Thus, it appears that this approach is susceptible of improving the identification of leukaemic lymphoblasts, and may be extended, by means of appropriate antisera, to the acute leukaemias in general.
当来自急性淋巴细胞白血病的白血病淋巴母细胞与动物抗人淋巴细胞球蛋白（AHLG）反应，随后与适当的异硫氰酸荧光素偶联抗体反应时，可观察到明亮的膜荧光，该荧光可持续到5个对数滴度，在更高稀释度时从环状变为斑点状。使用新鲜悬浮液和入射光可获得最佳结果。通过用慢性和急性髓细胞白血病的白细胞悬液反复预吸收AHLG，可以消除未吸收抗血清与成熟和未成熟髓细胞的交叉反应。因此，看来这种方法易于改进白血病淋巴母细胞的鉴定，并且通过适当的抗血清，可能推广到一般的急性白血病。
