Kittiwatanapaisan W, Wilairat P, Kotchabhakdi N, Govitrapong P
Neuro- and Behavioural Biology Center, Mahidol University, Nakornpathom, Thailand.
J Nutr Sci Vitaminol (Tokyo). 1993 Feb;39(1):81-8. doi: 10.3177/jnsv.39.81.
To demonstrate the role of vitamin E on cerebellar function, studies on rabbits fed low and high levels of dietary vitamin E were performed. The L-[3H]glutamate binding to cerebellar membranes of rabbits fed normal, high and low vitamin E diet showed receptor density (Bmax) values (mean +/- SEM) of 274 +/- 13, 637 +/- 37, and 265 +/- 60 pmol/mg protein, respectively, and dissociation equilibrium constant (KD) values of 257 +/- 99, 233 +/- 77, and 120 +/- 15 nM, respectively. Significant difference of Bmax from control was observed in the high dietary vitamin E group and of KD from control for the low dietary vitamin E group. These results indicate that dietary vitamin E levels have demonstrable effects on the central nervous system, especially the glutamate neurotransmitter system in rabbit cerebellum.
为了证明维生素E对小脑功能的作用，对喂食低水平和高水平膳食维生素E的兔子进行了研究。喂食正常、高维生素E和低维生素E饮食的兔子小脑血管膜上的L-[3H]谷氨酸结合显示，受体密度（Bmax）值（平均值±标准误）分别为274±13、637±37和265±60 pmol/mg蛋白质，解离平衡常数（KD）值分别为257±99、233±77和120±15 nM。高膳食维生素E组的Bmax与对照组有显著差异，低膳食维生素E组的KD与对照组有显著差异。这些结果表明，膳食维生素E水平对中枢神经系统有明显影响，尤其是对兔小脑的谷氨酸神经递质系统。
