Dahl C, Sahlén G, Grawé J, Johannisson A, Amneus H
Department of Zoology, Uppsala University, Sweden.
J Med Entomol. 1993 May;30(3):537-43. doi: 10.1093/jmedent/30.3.537.
Second and fourth instars of Culex quinquefasciatus Say and Aedes aegypti (L.) and fourth instars of Culiseta morsitans (Theobald) were fed five size classes of latex particles ranging from 0.56 to 5.75 microns. Each experimental run was done on two size classes of particles mixed in different proportions of 10:90, 50:50, and 90:10. Both the gut contents and the food mixtures were analyzed by flow cytometry, and the particle percentage composition was determined. The null hypothesis that no particle selection existed among instars or species was rejected for all species. Small instars ingested disproportionally more large particles in all suspensions. Cs. morsitans and Cx. quinquefasciatus showed variability in their responses. Ae. aegypti almost always ingested more of the larger particles. Differences in particle size selection were related to head morphology and feeding mode. Under experimental conditions, two parameters influenced particle size selection: the relative amount of small particles offered and the size difference between small and large particles. Flow cytometry analyses are recommended for quantitative studies of field material.
将致倦库蚊（Say）和埃及伊蚊（L.）的二龄和四龄幼虫以及骚扰库蚊（Theobald）的四龄幼虫喂食五种尺寸等级的乳胶颗粒，尺寸范围为0.56至5.75微米。每次实验对两种尺寸等级的颗粒以10:90、50:50和90:10的不同比例混合进行。通过流式细胞术分析肠道内容物和食物混合物，并确定颗粒百分比组成。所有物种均拒绝接受幼虫或物种之间不存在颗粒选择的零假设。在所有悬浮液中，小龄幼虫摄入的大颗粒比例过高。骚扰库蚊和致倦库蚊的反应存在差异。埃及伊蚊几乎总是摄入更多较大的颗粒。颗粒大小选择的差异与头部形态和摄食方式有关。在实验条件下，两个参数影响颗粒大小选择：提供的小颗粒的相对量以及小颗粒和大颗粒之间的尺寸差异。建议使用流式细胞术分析对野外材料进行定量研究。
